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Validation of an allele-specific marker associated with dehydration stress tolerance in a core set of foxtail millet accessions

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Title Validation of an allele-specific marker associated with dehydration stress tolerance in a core set of foxtail millet accessions
 
Creator Lata, Charu
Prasad, Manoj
 
Subject allele-specific marker
dehydration stress
foxtail millet
marker-aided breeding
SiDREB2
single- marker regression
single nucleotide polymorphism
 
Description Accepted date: April 22, 2012
Dehydration responsive element binding (DREB) genes play an important role in improving and enhancing stress tolerance of plants in response to abiotic stresses by interacting with DRE/CRT cis- element present in the promoter region of various stress-responsive genes. Thus, characterization of these genes and development of functional markers for effective alleles is important for crop improvement programmes. Identification of a synonymous single nucleotide polymorphism associated with dehydration tolerance at 558th bp (an A/G transition) in the SiDREB2 gene of foxtail millet (Setaria italica L.) and development of an allele-specific marker (ASM) has previously been reported by us. Here, we report on validation of the ASM in a core set of 170 foxtail millet accessions through single-marker regression analysis. The regression of lipid peroxidation (LP) on the ASM suggested that the SiDREB2 associated trait contributed to 27.18% of the total variation in LP among the 170 accessions indicating it to be a major QTL for dehydration tolerance. Thus, this ASM can be used for marker-aided breeding and allele-mining for dehydration tolerance in foxtail millet.
This study was supported by the Department of Biotechnology (Grant
No. BT/PR9851/AGR/02/521/2007), Govt. of India, New Delhi and
core grant from the National Institute of Plant Genome Research. We
are thankful to NBPGR, Hyderabad and UAS, GKVK, Bangalore,
India for providing the seed materials and Prof. Akhilesh K. Tyagi
(Director, NIPGR, New Delhi, India) for continuous support and
encouragement.
 
Date 2015-11-09T06:09:25Z
2015-11-09T06:09:25Z
2013
 
Type Article
 
Identifier Plant Breeding, 132(5): 496-499
1439-0523
http://172.16.0.77:8080/jspui/handle/123456789/344
http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0523.2012.01983.x/abstract
10.1111/j.1439-0523.2012.01983.x
 
Language en_US
 
Publisher John Wiley & Sons