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Catharanthus roseus mitogen-activated protein kinase 3 confers UV and heat tolerance to Saccharomyces cerevisiae

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Title Catharanthus roseus mitogen-activated protein kinase 3 confers UV and heat tolerance to Saccharomyces cerevisiae
 
Creator Raina, Susheel Kumar
Wankhede, Dhammaprakash Pandhari
Sinha, Alok Krishna
 
Subject Catharanthus roseus
CrMPK3
abiotic stress
UV
heat
cold
Saccharomyces cerevisiae
 
Description Accepted date: 30 Oct 2012
Catharanthus roseus is an important source of pharmaceutically important Monoterpenoid Indole Alkaloids (MIAs). Accumulation of many of the MIAs is induced in response to abiotic stresses such as wound, ultra violet (UV) irradiations, etc. Recently, we have demonstrated a possible role of CrMPK3, a C. roseus mitogen-activated protein kinase in stress-induced accumulation of a few MIAs. Here, we extend our findings using Saccharomyces cerevisiae to investigate the role of CrMPK3 in giving tolerance to abiotic stresses. Yeast cells transformed with CrMPK3 was found to show enhanced tolerance to UV and heat stress. Comparison of CrMPK3 and SLT2, a MAPK from yeast shows high-sequence identity particularly at conserved domains. Additionally, heat stress is also shown to activate a 43 kDa MAP kinase, possibly CrMPK3 in C. roseus leaves. These findings indicate the role of CrMPK3 in stress-induced MIA accumulation as well as in stress tolerance.
We thank Dr. Debasis Chattopadhyay, NIPGR, New Delhi for
providing yeast strain BCY123 and pYES2.1 vector. S.K.R. and
D.P.W. thank Council of Scientific and Industrial Research and
University Grant Commission, India for research fellowships.
This work is supported by core grant of National Institute of
Plant Genome Research, New Delhi, India from Department of
Biotechnology, India.
 
Date 2015-11-16T06:09:42Z
2015-11-16T06:09:42Z
2013
 
Type Article
 
Identifier Plant Signal. Behav., 8(1): 116-119
1559-2324
http://172.16.0.77:8080/jspui/handle/123456789/358
http://www.tandfonline.com/doi/abs/10.4161/psb.22716
10.4161/psb.22716
 
Language en_US
 
Publisher Taylor & Francis