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N-acetylglucosamine kinase, HXK1 is involved in morphogenetic transition and metabolic gene expression in Candida albicans

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Title N-acetylglucosamine kinase, HXK1 is involved in morphogenetic transition and metabolic gene expression in Candida albicans
 
Creator Rao, Kongara Hanumantha
Ghosh, Swagata
Natarajan, Krishnamurthy
Datta, Asis
 
Subject Candida albicans
HXK1
Morphogenetic Transition
Metabolic Gene Expression
HXK1
 
Description Accepted date: December 3, 2012
Candida albicans, a common fungal pathogen which diverged from the baker’s yeast Saccharomyces cerevisiae has the unique ability to utilise N-acetylglucosamine, an amino sugar and exhibits phenotypic differences. It has acquired intricate regulatory mechanisms at different levels in accordance with its life style. N-acetylglucosamine kinase, a component of the N-acetylglucosamine catabolic cascade is an understudied gene since Saccharomyces cerevisiae lacks it. We report HXK1 to act as both positive and negative regulator of transcription of genes involved in maintaining cellular homeostasis. It is involved in repression of hyphal specific genes in addition to metabolic genes. Its regulation of filamentation and GlcNAc metabolism is independent of the known classical regulators like EFG1, CPH1, RAS1, TPK2 or TUP1. Moreover, Hxk1-GFP is localised to cytoplasm, nucleus and mitochondria in a condition specific manner. By employing two-step affinity purification, we report the interaction of HXK1 with SIR2 under filamentation inducing conditions. Our work highlights a novel regulatory mechanism involved in filamentation repression and attempts to decipher the GlcNAc catabolic regulatory cascade in eukaryotes.
This study was funded by Indian Council of Medical Research (ICMR, project-file number 5/3/3/16/2007-ECD-1), Government of India and Professor of
Eminence Scheme of NIPGR, Department of Biotechnology, Ministry of Science & Technology, Government of India. K.H.R and S.G. received financial assistance
from CSIR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
 
Date 2015-11-16T06:29:33Z
2015-11-16T06:29:33Z
2013
 
Type Article
 
Identifier PLoS One, 8(1): e53638
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/360
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0053638
10.1371/journal.pone.0053638
 
Language en_US
 
Publisher PLOS