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Differentially expressed myo-inositol monophosphatase gene (CaIMP) in chickpea (Cicer arietinum L.) encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity and improves seed germination and seedling growth under abiotic stresses

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Title Differentially expressed myo-inositol monophosphatase gene (CaIMP) in chickpea (Cicer arietinum L.) encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity and improves seed germination and seedling growth under abiotic stresses
 
Creator Saxena, Saurabh C.
Salvi, Prafull
Kaur, Harmeet
Verma, Pooja
Petla, Bhanu Prakash
Rao, Venkateswara
Kamble, Nitin
Majee, Manoj
 
Subject Ascorbate
gluconeogenesis
inositol
multifunctional
phosphatase
seed germination
stress tolerance
 
Description Accepted date: 5 September 2013
myo-Inositol monophosphatase (IMP) is an essential enzyme in the myo-inositol metabolic pathway where it primarily dephosphorylates myo-inositol 1-phosphate to maintain the cellular inositol pool which is important for many metabolic and signalling pathways in plants. The stress-induced increased accumulation of inositol has been reported in a few plants including chickpea; however, the role and regulation of IMP is not well defined in response to stress. In this work, it has been shown that IMP activity is distributed in all organs in chickpea and was noticeably enhanced during environmental stresses. Subsequently, using degenerate oligonucleotides and RACE strategy, a full-length IMP cDNA (CaIMP) was cloned and sequenced. Biochemical study revealed that CaIMP encodes a lithium-sensitive phosphatase enzyme with broad substrate specificity, although maximum activity was observed with the myo-inositol 1-phosphate and l-galactose 1-phosphate substrates. Transcript analysis revealed that CaIMP is differentially expressed and regulated in different organs, stresses and phytohormones. Complementation analysis in Arabidopsis further confirmed the role of CaIMP in l-galactose 1-phosphate and myo-inositol 1-phosphate hydrolysis and its participation in myo-inositol and ascorbate biosynthesis. Moreover, Arabidopsis transgenic plants over-expressing CaIMP exhibited improved tolerance to stress during seed germination and seedling growth, while the VTC4/IMP loss-of-function mutants exhibited sensitivity to stress. Collectively, CaIMP links various metabolic pathways and plays an important role in improving seed germination and seedling growth, particularly under stressful environments.
This work was supported by the Department of
Biotechnology, Government of India under the scheme of
the ‘Next generation Challenge Programme in Chickpea
Genomics’ (Grant no: BT/PR12919/AGR/02/676/2009).
PS, HK, BP, VR, NK, and PV thank the Council of
Scientific and Industrial Research and the University
Grant Commission, Government of India, for research fellowships. We are grateful to Dr Glenda Gillaspy, Virginia Tech. USA for providing us with the vtc4 mutants (vtc4-3:SAIL_843_G10 and vtc4-4:SALK_077222).
 
Date 2015-11-18T07:09:24Z
2015-11-18T07:09:24Z
2013
 
Type Article
 
Identifier J. Exp. Bot., 64(18): 5623-5639
1460-2431
http://172.16.0.77:8080/jspui/handle/123456789/363
http://jxb.oxfordjournals.org/content/64/18/5623.long
10.1093/jxb/ert336
 
Language en_US
 
Publisher Oxford University Press