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Modified expression of an auxin-responsive rice CC-type glutaredoxin gene affects multiple abiotic stress responses
NIPGR Digital Knowledge Repository (NDKR)
View Archive Info| Field | Value | |
| Title |
Modified expression of an auxin-responsive rice CC-type glutaredoxin gene affects multiple abiotic stress responses
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| Creator |
Sharma, Raghvendra
Priya, Pushp Jain, Mukesh |
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| Subject |
Abiotic stress tolerance
Glutaredoxin Rice RNAi Transgenic plant |
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| Description |
Accepted date: 17 July 2013
Glutaredoxins (GRXs) are the ubiquitous oxidoreductase enzymes, which play an important role in defense against various stresses. Here, we report the role of a CC-type GRX gene from rice, OsGRX8, in abiotic stress tolerance. OsGRX8 protein was found to be localized in nucleus and cytosol and its gene expression is induced by various stress conditions and plant hormone auxin. The over-expression of OsGRX8 in Arabidopsis plants conferred reduced sensitivity to auxin and stress hormone, abscisic acid. In addition, the transgenic Arabidopsis plants exhibited enhanced tolerance to various abiotic stresses, including salinity, osmotic and oxidative stress. Further, the transgenic RNAi rice plants exhibited increased susceptibility to various abiotic stresses, which further confirmed the role of OsGRX8 in abiotic stress responses. The microarray data analysis revealed that expression of a large number of auxin-responsive, known stress-associated and transcription factor encoding genes was altered in GRX transgenic Arabidopsis plants in response to exogenous auxin and stress conditions as compared to wild-type plants. Altogether, these findings suggest the role of OsGRX8 in regulating abiotic stress response and may be used to engineer stress tolerance in crop plants. We acknowledge the financial support from the Department of Biotechnology, Government of India, under Innovative Young Biotechnologists Award scheme (grant numbers BT/BI/12/040/2005 and BT/02/IYBA/2010) and via core grant to NIPGR. Authors are thankful to anonymous reviewers for useful suggestions to improve the MS. |
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| Date |
2015-11-18T08:27:53Z
2015-11-18T08:27:53Z 2013 |
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| Type |
Article
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| Identifier |
Planta, 238(5): 871-884
1432-2048 http://172.16.0.77:8080/jspui/handle/123456789/366 http://link.springer.com/article/10.1007%2Fs00425-013-1940-y 10.1007/s00425-013-1940-y |
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| Language |
en_US
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| Publisher |
Springer
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