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The lld mutation in Pisum sativum used as a genetic tool to discern the plant leaflet/leaf developmental process

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Title The lld mutation in Pisum sativum used as a genetic tool to discern the plant leaflet/leaf developmental process
 
Creator Sharma, Vishakha
Tripathi, Bhumi Nath
Kumar, Sushil
 
Subject Adaxial-abaxial differentiation
Cup shaped leaf
Leaf development mechanism
Leaf vein network
Mediolateral polarity
Proximodistal growth
 
Description Leaves of P. sativum the double mutant genotype tendril-less (tl) leaflet-development (lld), due to the action of lld mutation, produce many leaflets that are aborted at different stages of development. Morphological, vein pattern and histological observations showed that aborted leaflets became cup/bell/trumpet (cup) shaped because of segmental differentiation in the leaflet primordium. Cup's inside lamina surface was adaxial and outer surfaces of cup and its stem were abaxial. The lld cups were phenotypically homologous to aborted leaves described in Arabidopsis thaliana mutants, angustifolia and those which underexpressed the HD-ZIP III proteins. Leaflet primordium was found to grow and establish three dimensional polarities apex-downwards. Primordium produced lateral outgrowth on one side of midvein. Differentiation, in the outgrowth of secondary veins, whose xylem tissues faced each other, established the adaxial-abaxial polarity. Lateral outgrowth then developed a cavity which got bounded by future adaxial epidermis. Further growth, veinlet formation, differentiation of palisade parenchyma and spongy parenchyma followed. Opening of lateral outgrowth at its outer midline produced a flat leaflet with lateral lamina spans. The structural and functional correspondence between leaflet and simple leaves suggested commonality between leaf and leaflet development mechanisms. A molecular model for the lld led leaflet abortion was also provided.
Thanks are due to Indian National Science
Academy, New Delhi and Council of Scientific and
Industrial Research, New Delhi for grant of scientistship schemes to SK, to SKA Institution for Research,
Education and Development for grant of postgraduate
fellowships to VS, to the Director NIPGR for
facilities, to RK Mishra for help in scanning of
leaves and to Vinod Kumar for help in field work.
 
Date 2015-11-18T09:02:25Z
2015-11-18T09:02:25Z
2013
 
Type Article
 
Identifier Indian J. Exp. Biol., 51(6): 421-434
0975-1009
http://172.16.0.77:8080/jspui/handle/123456789/369
http://nopr.niscair.res.in/handle/123456789/18669
 
Language en_US
 
Publisher NISCAIR-CSIR, India