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Interaction between two rice mitogen activated protein kinases and its possible role in plant defense

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Title Interaction between two rice mitogen activated protein kinases and its possible role in plant defense
 
Creator Sheikh, Arsheed H
Raghuram, Badmi
Jalmi, Siddhi K
Wankhede, Dhammaprakash P
Singh, Pallavi
Sinha, Alok Krishna
 
Subject MAP kinase
Protein-protein interaction
Pseudomonas syringae pv. tabaci
Rice
Yeast two-hybrid assay
 
Description Accepted date: 24 August 2013
Background: The canonical mitogen activated protein kinase (MAPK) signaling pathway plays a vital role in
carrying out the normal growth and development of the plant. The pathway, connecting the upstreams signal with
the downstream target is considered to be linear, mostly starting with a MAPKKK and ending in a MAPK.
Results: Here we report a novel interaction between two rice MAPKs, OsMPK20-4 and OsMPK3 suggesting the
complex nature of the pathway rather than a linear one at individual steps. The interaction between OsMPK20-4
and OsMPK3 found by yeast two-hybrid analysis was confirmed in planta by co-immunoprecipitation and
fluorescence resonance energy transfer (FRET) assays. The interaction is specific and is phosphorylation
independent. The results suggest a role of the interaction between OsMPK20-4 and OsMPK3 in basic plant defense.
Conclusions: The current novel work showing the physical interaction between two plant MAPKs, OsMPK20-4 and
OsMPK3 is the diversion from the dogma of a typical MAPK cascade thereby opening a new dimension to the
MAPK signal transduction.
Authors acknowledge Prof. T. Roitsch, University of Graz, Austria for providing
Pseudomonas syringae pv. tabaci strain. Technical help provided by the
Confocal microscopy facility of NIPGR is kindly acknowledged. AHS and PS
acknowledge Council of Scientific and Industrial Research, India; BR and SKJ
acknowledge Department of Biotechnology, India; DPW acknowledges
University Grant Commission and National Institute of Plant Genome
Research, India for fellowship. The work is supported by core grant of
National Institute of Plant Genome Research from Department of
Biotechnology, New Delhi, India.
 
Date 2015-11-19T07:16:52Z
2015-11-19T07:16:52Z
2013
 
Type Article
 
Identifier BMC Plant Biol., 13: 121
1471-2229
http://172.16.0.77:8080/jspui/handle/123456789/370
http://www.biomedcentral.com/1471-2229/13/121
10.1186/1471-2229-13-121
 
Language en_US
 
Publisher BioMed Central Ltd