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Comprehensive genomic analysis and expression profiling of phospholipase C gene family during abiotic stresses and development in rice

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Title Comprehensive genomic analysis and expression profiling of phospholipase C gene family during abiotic stresses and development in rice
 
Creator Singh, Amarjeet
Kanwar, Poonam
Pandey, Amita
Tyagi, Akhilesh K.
Sopory, Sudhir K.
Kapoor, Sanjay
Pandey, Girdhar K.
 
Subject Abiotic Stresses
Rice
Genomic Analysis
Expression Profiling
Phospholipase C
 
Description Accepted date: March 22, 2013
BACKGROUND:

Phospholipase C (PLC) is one of the major lipid hydrolysing enzymes, implicated in lipid mediated signaling. PLCs have been found to play a significant role in abiotic stress triggered signaling and developmental processes in various plant species. Genome wide identification and expression analysis have been carried out for this gene family in Arabidopsis, yet not much has been accomplished in crop plant rice.
METHODOLOGY/PRINCIPAL FINDINGS:

An exhaustive in-silico exploration of rice genome using various online databases and tools resulted in the identification of nine PLC encoding genes. Based on sequence, motif and phylogenetic analysis rice PLC gene family could be divided into phosphatidylinositol-specific PLCs (PI-PLCs) and phosphatidylcholine- PLCs (PC-PLC or NPC) classes with four and five members, respectively. A comparative analysis revealed that PLCs are conserved in Arabidopsis (dicots) and rice (monocot) at gene structure and protein level but they might have evolved through a separate evolutionary path. Transcript profiling using gene chip microarray and quantitative RT-PCR showed that most of the PLC members expressed significantly and differentially under abiotic stresses (salt, cold and drought) and during various developmental stages with condition/stage specific and overlapping expression. This finding suggested an important role of different rice PLC members in abiotic stress triggered signaling and plant development, which was also supported by the presence of relevant cis-regulatory elements in their promoters. Sub-cellular localization of few selected PLC members in Nicotiana benthamiana and onion epidermal cells has provided a clue about their site of action and functional behaviour.
CONCLUSION/SIGNIFICANCE:

The genome wide identification, structural and expression analysis and knowledge of sub-cellular localization of PLC gene family envisage the functional characterization of these genes in crop plants in near future.
The authors acknowledge Department of Biotechnology (DBT), Department of Science and Technology (DST) and Council for Scientific and Industrial Research (CSIR), INDIA for funding the research in GKP's lab. AS and PK acknowledge senior research fellowship from CSIR, India. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
 
Date 2015-11-19T08:42:00Z
2015-11-19T08:42:00Z
2013
 
Type Article
 
Identifier PLoS One, 8(4): e62494
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/372
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0062494
10.1371/journal.pone.0062494
 
Language en_US
 
Publisher PLOS