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Phosphoproteomic dynamics of chickpea (Cicer arietinum L.) reveals shared and distinct components of dehydration response

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Title Phosphoproteomic dynamics of chickpea (Cicer arietinum L.) reveals shared and distinct components of dehydration response
 
Creator Subba, Pratigya
Barua, Pragya
Kumar, Rajiv
Datta, Asis
Soni, Kamlesh Kumar
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject dehydration response
food legume
IMAC
mass spectrometry
post-translational modification
phosphorylome dynamics
 
Description Reversible protein phosphorylation is a ubiquitous regulatory mechanism that plays critical roles in transducing stress signals to bring about coordinated intracellular responses. To gain better understanding of dehydration response in plants, we have developed a differential phosphoproteome in a food legume, chickpea (Cicer arietinum L.). Three-week-old chickpea seedlings were subjected to progressive dehydration by withdrawing water, and the changes in the phosphorylation status of a large repertoire of proteins were monitored. The proteins were resolved by 2-DE and stained with phosphospecific fluorescent Pro-Q Diamond dye. Mass spectrometric analysis led to the identification of 91 putative phosphoproteins, presumably involved in a variety of functions including cell defense and rescue, photosynthesis and photorespiration, molecular chaperones, and ion transport, among others. Multiple sites of phosphorylation were predicted on several key elements, which include both the regulatory as well as the functional proteins. A critical survey of the phosphorylome revealed a DREPP (developmentally regulated plasma membrane protein) plasma membrane polypeptide family protein, henceforth designated CaDREPP1. The transcripts of CaDREPP1 were found to be differentially regulated under dehydration stress, further corroborating the proteomic results. This work provides new insights into the possible phosphorylation events triggered by the conditions of progressive water-deficit in plants.
This work was supported by grant [BT/PR/10677/PBD16/795]
from the Department of Biotechnology (DBT), Govt. of India. We
thank the CSIR and DBT for providing research fellowship to P.S.,
P.B., and R.K., respectively. We also thank International Crops
Research Institute for the Semi-Arid Tropics (ICRISAT),
Hyderabad, India for providing chickpea seeds. Mr. Jasbeer
Singh is thanked for illustrations and graphical representation in
the manuscript.
 
Date 2015-11-24T06:26:11Z
2015-11-24T06:26:11Z
2013
 
Type Article
 
Identifier J. Proteome Res., 12(11): 5025-5047
1535-3893
http://172.16.0.77:8080/jspui/handle/123456789/375
http://pubs.acs.org/doi/abs/10.1021/pr400628j
10.1021/pr400628j
 
Language en_US
 
Publisher American Chemical Society