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Characterization of the nuclear proteome of a dehydration-sensitive cultivar of chickpea and comparative proteomic analysis with a tolerant cultivar

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Title Characterization of the nuclear proteome of a dehydration-sensitive cultivar of chickpea and comparative proteomic analysis with a tolerant cultivar
 
Creator Subba, Pratigya
Kumar, Rajiv
Gayali, Saurabh
Shekhar, Shubhendu
Parveen, Shaista
Pandey, Aarti
Datta, Asis
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Adaptive strategies
Comparative proteomics
Dehydration
Nuclei-enriched fraction
Pulse legume
ROS catabolising enzymes
 
Description Accepted date: March 19, 2013
Water deficit or dehydration hampers plant growth and development, and shrinks harvest size of major crop species worldwide. Therefore, a better understanding of dehydration response is the key to decipher the regulatory mechanism of better adaptation. In recent years, nuclear proteomics has become an attractive area of research, particularly to study the role of nucleus in stress response. In this study, a proteome of dehydration-sensitive chickpea cultivar (ICCV-2) was generated from nuclei-enriched fractions. The LC-MS/MS analysis led to the identification of 75 differentially expressed proteins presumably associated with different metabolic and regulatory pathways. Nuclear localisation of three candidate proteins was validated by transient expression assay. The ICCV-2 proteome was then compared with that of JG-62, a tolerant cultivar. The differential proteomics and in silico analysis revealed cultivar-specific differential expression of many proteins involved in various cellular functions. The differential tolerance could be attributed to altered expression of many structural proteins and the proteins involved in stress adaptation, notably the ROS catabolising enzymes. Further, a comprehensive comparison on the abiotic stress-responsive nuclear proteome was performed using the datasets published thus far. These findings might expedite the functional determination of the dehydration-responsive proteins and their prioritisation as potential molecular targets for better adaptation.
This work was supported by grants (BT/PR/10677/
PBD/16/795) from the Department of Biotechnology (DBT),
Government of India. We thank the Council of Scientific and Industrial Research (CSIR), Government of India and the DBT for
providing predoctoral fellowship to PS, RK, SG and SP. We thank
Dr. Suchismita Dass for critical reading of the manuscript and
Mr. Jasbeer Singh for illustrations and graphical representation
in the manuscript.
 
Date 2015-11-24T08:42:03Z
2015-11-24T08:42:03Z
2013
 
Type Article
 
Identifier Proteomics, 13(12-13): 1973-1992
1615-9861
http://172.16.0.77:8080/jspui/handle/123456789/376
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201200380/abstract
10.1002/pmic.201200380
 
Language en_US
 
Publisher John Wiley & Sons Ltd