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Arabidopsis CAM7 and HY5 physically interact and directly bind to the HY5 promoter to regulate its expression and thereby promote photomorphogenesis

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Title Arabidopsis CAM7 and HY5 physically interact and directly bind to the HY5 promoter to regulate its expression and thereby promote photomorphogenesis
 
Creator Abbas, Nazia
Maurya, Jay P.
Senapati, Dhirodatta
Gangappa, Sreeramaiah N.
Chattopadhyay, Sudip
 
Subject Arabidopsis thaliana
CALMODULIN7
HYPOCOTYL5
Photomorphogenesis
 
Description Accepted date: February 12, 2014
Arabidopsis thaliana CALMODULIN7 (CAM7), a unique member of the calmodulin gene family, plays a crucial role as a transcriptional regulator in seedling development. The elongated HYPOCOTYL5 (HY5) bZIP protein, an integrator of multiple signaling pathways, also plays an important role in photomorphogenic growth and light-regulated gene expression. CAM7 acts synergistically with HY5 to promote photomorphogenesis at various wavelengths of light. Although the genetic relationships between CAM7 and HY5 in light-mediated seedling development have been demonstrated, the molecular connectivity between CAM7 and HY5 is unknown. Furthermore, whereas HY5-mediated gene regulation has been fairly well investigated, the transcriptional regulation of HY5 is largely unknown. Here, we report that HY5 expression is regulated by HY5 and CAM7 at various wavelengths of light and also at various stages of development. In vitro and in vivo DNA-protein interaction studies suggest that HY5 and CAM7 bind to closely located T/G- and E-box cis-acting elements present in the HY5 promoter, respectively. Furthermore, CAM7 and HY5 physically interact and regulate the expression of HY5 in a concerted manner. Taken together, these results demonstrate that CAM7 and HY5 directly interact with the HY5 promoter to mediate the transcriptional activity of HY5 during Arabidopsis seedling development.
We thank Hathi Ram (EMBL Heidelberg) for critically reading and
commenting on the article. We thank Xing Wang Deng (Yale University)
for providing us with the seeds of the HY5OE transgenic line. This work was supported by a J.C. Bose National Fellowship Award Grant from the
Department of Science and Technology, Government of India, by
a National Institute of Plant Genome Research internal grant to S.C.,
and by the following fellowships: from the Department of Biotechnology,
Government of India, to N.A., from the Council of Scientific and Industrial
Research, Government of India, to J.P.M., and from the National Institute
of Technology to D.S.
 
Date 2015-11-26T09:05:47Z
2015-11-26T09:05:47Z
2014
 
Type Article
 
Identifier Plant Cell, 26(3): 1036-1052
1532-298X
http://172.16.0.77:8080/jspui/handle/123456789/388
http://www.plantcell.org/content/26/3/1036.long
10.1105/tpc.113.122515
 
Language en_US
 
Publisher American Society of Plant Biologists