Record Details

Expanding frontiers in plant transcriptomics in aid of functional genomics and molecular breeding

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Expanding frontiers in plant transcriptomics in aid of functional genomics and molecular breeding
 
Creator Agarwal, Pinky
Parida, Swarup K.
Mahto, Arunima
Das, Sweta
Mathew, Iny Elizebeth
Malik, Naveen
Tyagi, Akhilesh K.
 
Subject Functional genomics
Microarray
NGS
RNA-seq
Transcriptome
 
Description Accepted date: 01 OCT 2014
The transcript pool of a plant part, under any given condition, is a collection of mRNAs that will pave the way for a biochemical reaction of the plant to stimuli. Over the past decades, transcriptome study has advanced from Northern blotting to RNA sequencing (RNA-seq), through other techniques, of which real-time quantitative polymerase chain reaction (PCR) and microarray are the most significant ones. The questions being addressed by such studies have also matured from a solitary process to expression atlas and marker-assisted genetic enhancement. Not only genes and their networks involved in various developmental processes of plant parts have been elucidated, but also stress tolerant genes have been highlighted. The transcriptome of a plant with altered expression of a target gene has given information about the downstream genes. Marker information has been used for breeding improved varieties. Fortunately, the data generated by transcriptome analysis has been made freely available for ample utilization and comparison. The review discusses this wide variety of transcriptome data being generated in plants, which includes developmental stages, abiotic and biotic stress, effect of altered gene expression, as well as comparative transcriptomics, with a special emphasis on microarray and RNA-seq. Such data can be used to determine the regulatory gene networks, which can subsequently be utilized for generating improved plant varieties.
A. M. and N. M. acknowledge the junior and senior
research fellowship, respectively, from Council for Scientific and Industrial Research (CSIR) and S. D. and I. E. M. acknowledge the senior research fellowship from University Grants Commission (UGC). P. A., S. K. P., and A. K. T. thank the Department of Biotechnology (DBT), India for grants supporting research.
 
Date 2015-11-26T09:36:59Z
2015-11-26T09:36:59Z
2014
 
Type Article
 
Identifier Biotechnol. J., 9(12): 1480-1492
1860-6768
http://172.16.0.77:8080/jspui/handle/123456789/390
http://onlinelibrary.wiley.com/doi/10.1002/biot.201400063/abstract
10.1002/biot.201400063
 
Language en_US
 
Publisher John Wiley & Sons Ltd