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Phylogenetic analysis reveals conservation and diversification of micro RNA166 genes among diverse plant species

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Title Phylogenetic analysis reveals conservation and diversification of micro RNA166 genes among diverse plant species
 
Creator Barik, Suvakanta
SarkarDas, Shabari
Singh, Archita
Gautam, Vibhav
Kumar, Pramod
Majee, Manoj
Sarkar, Ananda K.
 
Subject MicroRNA
miR166
Genome
Evolution
Plants
 
Description Accepted date: 12 November 2013
Similar to the majority of the microRNAs, mature miR166s are derived from multiple members of MIR166 genes (precursors) and regulate various aspects of plant development by negatively regulating their target genes (Class III HD-ZIP). The evolutionary conservation or functional diversification of miRNA166 family members remains elusive. Here, we show the phylogenetic relationships among MIR166 precursor and mature sequences from three diverse model plant species. Despite strong conservation, some mature miR166 sequences, such as ppt-miR166m, have undergone sequence variation. Critical sequence variation in ppt-miR166m has led to functional diversification, as it targets non-HD-ZIPIII gene transcript (s). MIR166 precursor sequences have diverged in a lineage specific manner, and both precursors and mature osa-miR166i/j are highly conserved. Interestingly, polycistronic MIR166s were present in Physcomitrella and Oryza but not in Arabidopsis. The nature of cis-regulatory motifs on the upstream promoter sequences of MIR166 genes indicates their possible contribution to the functional variation observed among miR166 species.
We thank DBT (Department of Biotechnology, India) and NIPGR (National Institute of Plant Genome Research)
for Ramalingaswami fellowship (BT/HRD/35/02/06/2008) to AKS and
internal funding (and facility) to AKS and MM respectively. The fellowship to AS, VG, and PK by CSIR (Council for Scientific and Industrial
Research, India) and to SS and SB by NIPGR is kindly acknowledged.
 
Date 2015-12-11T07:20:51Z
2015-12-11T07:20:51Z
2014
 
Type Article
 
Identifier Genomics, 103(1): 114-121
0888-7543
http://172.16.0.77:8080/jspui/handle/123456789/397
http://www.sciencedirect.com/science/article/pii/S0888754313002073
10.1016/j.ygeno.2013.11.004
 
Language en_US
 
Publisher Elsevier B.V.