Record Details

A multi-step phosphorelay two-component system impacts on tolerance against dehydration stress in common wheat

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title A multi-step phosphorelay two-component system impacts on tolerance against dehydration stress in common wheat
 
Creator Gahlaut, Vijay
Mathur, Saloni
Dhariwal, Raman
Khurana, Jitendra P.
Tyagi, Akhilesh K.
Balyan, Harindra S.
Gupta, Pushpendra K.
 
Subject Dehydration stress
In silico
Two-component signalling elements
qRT-PCR
 
Description Accepted date: 31 August 2014
Wheat is an important staple crop, and its productivity is severely constrained by drought stress (DS). An understanding of the molecular basis of drought tolerance is necessary for genetic improvement of wheat for tolerance to DS. The two-component system (TCS) serves as a common sensor-regulator coupling mechanism implicated in the regulation of diverse biological processes (including response to DS) not only in prokaryotes, but also in higher plants. In the latter, TCS generally consists of two signalling elements, a histidine kinase (HK) and a response regulator (RR) associated with an intermediate element called histidine phosphotransferase (HPT). Keeping in view the possible utility of TCS in developing water use efficient (WUE) wheat cultivars, we identified and characterized 62 wheat genes encoding TCS elements in a silico study; these included 7 HKs, 45 RRs along with 10 HPTs. Twelve of the 62 genes showed relatively higher alterations in the expression under drought. The quantitative RT-PCR (qRT-PCR)-based expression analysis of these 12 TCS genes was carried out in wheat seedlings of a drought sensitive (HD2967) and a tolerant (Dharwar Dry) cultivar subjected to either dehydration stress or cytokinin treatment. The expression of these 12 genes under dehydration stress differed in sensitive and tolerant genotypes, even though for individual genes, both showed either up-regulation or down-regulation. In response to the treatment of cytokinin, the expression of type-A RR genes was higher in the tolerant genotype, relative to that in the sensitive genotype, the situation being reverse for the type-B RRs. These results have been discussed in the context of the role of TCS elements in drought tolerance in wheat.
We thank The Head, Department of Genetics and
Plant Breeding, for providing facilities for undertaking some of the work
reported in this manuscript. The manuscript was written during the period
when PKG held the position of a NASI Senior Scientist awarded by the
National Academy of Sciences India (NASI). The facilities of BIF sponsored by Department of Biotechnology, Government of India were used for
in silico analysis. Part of this work was also financially supported by the
Department of Biotechnology, Government of India.
 
Date 2015-12-15T07:13:28Z
2015-12-15T07:13:28Z
2014
 
Type Article
 
Identifier Funct. Integr. Genomics, 14(4): 707-716
1438-7948
http://172.16.0.77:8080/jspui/handle/123456789/402
http://link.springer.com/article/10.1007%2Fs10142-014-0398-8
10.1007/s10142-014-0398-8
 
Language en_US
 
Publisher Springer