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Genomic survey, gene expression analysis and structural modeling suggest diverse roles of DNA methyltransferases in legumes

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Title Genomic survey, gene expression analysis and structural modeling suggest diverse roles of DNA methyltransferases in legumes
 
Creator Garg, Rohini
Kumari, Romika
Tiwari, Sneha
Goyal, Shweta
 
Subject DNA methylation
Legumes
Gene Expression Analysis
 
Description Accepted date: January 15, 2014
DNA methylation plays a crucial role in development through inheritable gene silencing. Plants possess three types of DNA methyltransferases (MTases), namely Methyltransferase (MET), Chromomethylase (CMT) and Domains Rearranged Methyltransferase (DRM), which maintain methylation at CG, CHG and CHH sites. DNA MTases have not been studied in legumes so far. Here, we report the identification and analysis of putative DNA MTases in five legumes, including chickpea, soybean, pigeonpea, Medicago and Lotus. MTases in legumes could be classified in known MET, CMT, DRM and DNA nucleotide methyltransferases (DNMT2) subfamilies based on their domain organization. First three MTases represent DNA MTases, whereas DNMT2 represents a transfer RNA (tRNA) MTase. Structural comparison of all the MTases in plants with known MTases in mammalian and plant systems have been reported to assign structural features in context of biological functions of these proteins. The structure analysis clearly specified regions crucial for protein-protein interactions and regions important for nucleosome binding in various domains of CMT and MET proteins. In addition, structural model of DRM suggested that circular permutation of motifs does not have any effect on overall structure of DNA methyltransferase domain. These results provide valuable insights into role of various domains in molecular recognition and should facilitate mechanistic understanding of their function in mediating specific methylation patterns. Further, the comprehensive gene expression analyses of MTases in legumes provided evidence of their role in various developmental processes throughout the plant life cycle and response to various abiotic stresses. Overall, our study will be very helpful in establishing the specific functions of DNA MTases in legumes.
RG is thankful to Prof. A.K. Tyagi for reading of the MS. We are also
thankful to Dr. Mukesh Jain, NIPGR, for providing facility to carry out
real-time PCR and subcellular localization experiments and critical reading
of the MS.
 
Date 2015-12-15T07:28:23Z
2015-12-15T07:28:23Z
2014
 
Type Article
 
Identifier PLoS One, 9(2): e88947
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/403
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088947
10.1371/journal.pone.0088947
 
Language en_US
 
Publisher PLOS