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Deep transcriptome sequencing of wild halophyte rice, Porteresia coarctata, provides novel insights into the salinity and submergence tolerance factors

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Title Deep transcriptome sequencing of wild halophyte rice, Porteresia coarctata, provides novel insights into the salinity and submergence tolerance factors
 
Creator Garg, Rohini
Verma, Mohit
Agrawal, Shashank
Shankar, Rama
Majee, Manoj
Jain, Mukesh
 
Subject metabolic pathways
Porteresia
salinity tolerance
submergence
transcriptome analysis
 
Description Accepted date: 6 September 2013
Porteresia coarctata is a wild relative of rice with capability of high salinity and submergence tolerance. The transcriptome analyses of Porteresia can lead to the identification of candidate genes involved in salinity and submergence tolerance. We sequenced the transcriptome of Porteresia under different conditions using Illumina platform and generated about 375 million high-quality reads. After optimized assembly, a total of 152 367 unique transcript sequences with average length of 794 bp were obtained. Many of these sequences might represent fragmented transcripts. Functional annotation revealed the presence of genes involved in diverse cellular processes and 2749 transcription factor (TF)-encoding genes in Porteresia. The differential gene expression analyses identified a total of 15 158 genes involved in salinity and/or submergence response(s). The stress-responsive members of different TF families, including MYB, bHLH, AP2-EREBP, WRKY, bZIP and NAC, were identified. We also revealed key metabolic pathways, including amino acid biosynthesis, hormone biosynthesis, secondary metabolite biosynthesis, carbohydrate metabolism and cell wall structures, involved in stress tolerance in Porteresia. The transcriptome analyses of Porteresia are expected to highlight genes/pathways involved in salinity and submergence tolerance of this halophyte species. The data can serve as a resource for unravelling the underlying mechanism and devising strategies to engineer salinity and submergence tolerance in rice.
This work was financially supported by the core grant
from NIPGR. R.G. acknowledges INSPIRE Faculty Award
from the Department of Science and Technology and Innovative Young Biotechnologists Award from the
Department of Biotechnology, Government of India.
R.S. acknowledges the Department of Biotechnology,
Government of India, for research fellowship.
 
Date 2015-12-16T04:21:04Z
2015-12-16T04:21:04Z
2014
 
Type Article
 
Identifier DNA Res., 21(1): 69-84
1340-2838
http://172.16.0.77:8080/jspui/handle/123456789/405
http://dnaresearch.oxfordjournals.org/content/21/1/69
10.1093/dnares/dst042
 
Language en_US
 
Publisher Oxford University Press