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Identification and functional analysis of AG1-IA specific genes of Rhizoctonia solani

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Title Identification and functional analysis of AG1-IA specific genes of Rhizoctonia solani
 
Creator Ghosh, Srayan
Gupta, Santosh Kumar
Jha, Gopaljee
 
Subject Sheath blight disease
Fungal pathogenesis
Anastomosis group
Disease resistance
Host specificity
Necrotrophs
Secondary metabolism
Toxins
 
Description Accepted date: 10 July 2014
Rhizoctonia solani is an important necrotrophic fungal pathogen which causes disease on diverse plant species. It has been classified into 14 genetically distinct anastomosis groups (AGs), however, very little is known about their genomic diversity. AG1-IA causes sheath blight disease in rice and controlling this disease remains a challenge for sustainable rice cultivation. Recently the draft genome sequences of AG1-IA (rice isolate) and AG1-IB (lettuce isolate) had become publicly available. In this study, using comparative genomics, we report identification of 3,942 R. solani genes that are uniquely present in AG1-IA. Many of these genes encode important biological, molecular functions and exhibit dynamic expression during in-planta growth of the pathogen in rice. Based upon sequence similarity with genes that are required for plant and human/zoonotic diseases, we identified several putative virulence/pathogenicity determinants amongst AG1-IA specific genes. While studying the expression of 19 randomly selected genes, we identified three genes highly up-regulated during in-planta growth. The detailed in silico characterization of these genes and extent of their up-regulation in different rice genotypes, having variable degree of disease susceptibility, suggests their importance in rice-Rhizoctonia interactions. In summary, the present study reports identification, functional characterization of AG1-IA specific genes and predicts important virulence determinants that might enable the pathogen to grow inside hostile plant environment. Further characterization of these genes would shed useful insights about the pathogenicity mechanism of AG1-IA on rice.
SG was supported by SPM fellowship from
Council of Scientific and Industrial Research (Govt. of India) and
SKG was supported by Post-Doctoral Research fellowship from
Department of Biotechnology (DBT, Govt of India). We acknowledge Praveen Raj S, CromDx Solutions Pvt. Ltd for assistance in
computational analysis and central instrumentation facility at NIPGR
for sequencing and qRT-PCR analysis. We also thank Dr G.S. Laha,
DRR, Hyderabad for providing R. solani strains and AiPing Zheng,
SICAU, China and Daniel Wibberg, CeBiTec, Bielefield University,
Germany for making draft genome sequences of AG1-IA and AG1-B,
R. solani strains publicly available. This work was supported by core
research grant from the National Institute of Plant Genome Research, India. The funders had no role in study design, data collection and
analysis, decision to publish, or preparation of the manuscript.
 
Date 2015-12-16T05:06:23Z
2015-12-16T05:06:23Z
2014
 
Type Article
 
Identifier Current Genetics, 60(4): 327-341
1432-0983
http://172.16.0.77:8080/jspui/handle/123456789/407
http://link.springer.com/article/10.1007%2Fs00294-014-0438-x
10.1007/s00294-014-0438-x
 
Language en_US
 
Publisher Springer