Record Details

LeMYC2 acts as a negative regulator of blue light mediated photomorphogenic growth, and promotes the growth of adult tomato plants

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title LeMYC2 acts as a negative regulator of blue light mediated photomorphogenic growth, and promotes the growth of adult tomato plants
 
Creator Gupta, Nisha
Prasad, V Babu Rajendra
Chattopadhyay, Sudip
 
Subject photomorphogenic growth
tomato plants
Lycopersicon esculentum
 
Description Accepted date: 28 January 2014
Background:

Arabidopsis ZBF1/MYC2bHLH transcription factor is a repressor of photomorphogenesis, and acts as a point of cross talk in light, abscisic acid (ABA) and jasmonic acid (JA) signaling pathways. MYC2 also functions as a positive regulator of lateral root development and flowering time under long day conditions. However, the function of MYC2 in growth and development remains unknown in crop plants. Results: Here, we report the functional analyses of LeMYC2 in tomato (Lycopersicon esculentum). The amino acid
sequence of LeMYC2 showed extensive homology with Arabidopsis MYC2, containing the conserved bHLH domain.
To study the function of LeMYC2 in tomato, overexpression and RNA interference (RNAi) LeMYC2 tomato transgenic
plants were generated. Examination of seedling morphology, physiological responses and light regulated gene
expression has revealed that LeMYC2 works as a negative regulator of blue light mediated photomorphogenesis.
Furthermore, LeMYC2 specifically binds to the G-box of LeRBCS-3A promoter. Overexpression of LeMYC2 has led to
increased root length with more number of lateral roots. The tomato plants overexpressing LeMYC2 have reduced
internode distance with more branches, and display the opposite morphology to RNAi transgenic lines.
Furthermore, this study shows that LeMYC2 promotes ABA and JA responsiveness.
Conclusions: Collectively, this study highlights that working in light, ABA and JA signaling pathways LeMYC2 works
as an important regulator for growth and development in tomato plants.
We thank Sreeramiah N. Gangappa for critically reading and commenting on
this manuscript. This work is supported by a Research Grant from
Department of Biotechnology, Government of India, and NIPGR internal fund to SC; VBRP is recipient of fellowship from University Grants Commission,
Government of India.
 
Date 2015-12-16T05:33:46Z
2015-12-16T05:33:46Z
2014
 
Type Article
 
Identifier BMC Plant Biol., 14: 38
1471-2229
http://172.16.0.77:8080/jspui/handle/123456789/409
http://www.biomedcentral.com/1471-2229/14/38
10.1186/1471-2229-14-38
 
Language en_US
 
Publisher BioMed Central Ltd