Record Details

Insights into transcriptional regulation of β-D-N-acetylhexosaminidase, an N-glycan-processing enzyme involved in ripening-associated fruit softening

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Insights into transcriptional regulation of β-D-N-acetylhexosaminidase, an N-glycan-processing enzyme involved in ripening-associated fruit softening
 
Creator Irfan, Mohammad
Ghosh, Sumit
Kumar, Vinay
Chakraborty, Niranjan
Chakraborty, Subhra
Datta, Asis
 
Subject Fruit ripening
fruit ripening-specific promoter
β-Hex
RIN
SlASR1
transcriptional regulation
 
Description Accepted date: 2 July 2014
Tomato (Solanum lycopersicum) fruit ripening-specific N-glycan processing enzyme, β-D-N-acetylhexosaminidase (β-Hex), plays an important role in the ripening-associated fruit-softening process. However, the regulation of fruit ripening-specific expression of β-Hex is not well understood. We have identified and functionally characterized the fruit ripening-specific promoter of β-Hex and provided insights into its transcriptional regulation during fruit ripening. Our results demonstrate that RIPENING INHIBITOR (RIN), a global fruit ripening regulator, and ABSCISIC ACID STRESS RIPENING 1 (SlASR1), a poorly characterized ripening-related protein, are the transcriptional regulators of β-Hex. Both RIN and SlASR1 directly bound to the β-Hex promoter fragments containing CArG and C₂₋₃(C/G)A cis-acting elements, the binding sites for RIN and SlASR1, respectively. Moreover, β-Hex expression/promoter activity in tomato fruits was downregulated once expression of either RIN or SlASR1 was suppressed; indicating that RIN and SlASR1 positively regulate the transcription of β-Hex during fruit ripening. Interestingly, RIN could also bind to the SlASR1 promoter, which contains several CArG cis-acting elements, and SlASR1 expression was suppressed in rin mutant fruits, indicating that RIN also acts as a positive regulator of SlASR1 expression during fruit ripening. Taken together, these results suggest that RIN, both directly and indirectly, through SlASR1, regulates the transcription of β-Hex during fruit ripening. The fruit ripening-specific promoter of β-Hex could be a useful tool in regulating gene expression during fruit ripening.
This work was financially supported by a research
grant (BT/01/CEIB/12/II/01) from the Department of
Biotechnology and core research grant from the National
Institute of Plant Genome Research.
 
Date 2015-12-16T07:04:40Z
2015-12-16T07:04:40Z
2014
 
Type Article
 
Identifier J. Exp. Bot., 65(20): 5835-5848
1460-2431
http://172.16.0.77:8080/jspui/handle/123456789/414
http://jxb.oxfordjournals.org/content/65/20/5835
10.1093/jxb/eru324
 
Language en_US
 
Publisher Oxford University Press