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Membrane-associated proteomics of chickpea identifies Sad1/UNC-84 protein (CaSUN1), a novel component of dehydration signaling

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Title Membrane-associated proteomics of chickpea identifies Sad1/UNC-84 protein (CaSUN1), a novel component of dehydration signaling
 
Creator Jaiswal, Dinesh Kumar
Mishra, Poonam
Subba, Pratigya
Rathi, Divya
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Plant Stress Responses
Abiotic
Plant signalling
 
Description Accepted date: 07 February 2014
Dehydration affects almost all the physiological processes including those that result in the accumulation of misfolded proteins in the endoplasmic reticulum (ER), which in turn elicits a highly conserved signaling, the unfolded protein response (UPR). We investigated the dehydration-responsive membrane-associated proteome of a legume, chickpea, by 2-DE coupled with mass spectrometry. A total of 184 protein spots were significantly altered over a dehydration treatment of 120 h. Among the differentially expressed proteins, a non-canonical SUN domain protein, designated CaSUN1 (Cicer arietinum Sad1/UNC-84), was identified. CaSUN1 localized to the nuclear membrane and ER, besides small vacuolar vesicles. The transcripts were downregulated by both abiotic and biotic stresses, but not by abscisic acid treatment. Overexpression of CaSUN1 conferred stress tolerance in transgenic Arabidopsis. Furthermore, functional complementation of the yeast mutant, slp1, could rescue its growth defects. We propose that the function of CaSUN1 in stress response might be regulated via UPR signaling.
 
Date 2015-12-17T04:27:03Z
2015-12-17T04:27:03Z
2014
 
Type Article
 
Identifier Scientific Reports, 4: 4177
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/417
http://www.nature.com/articles/srep04177
10.1038/srep04177
 
Language en_US
 
Publisher Nature Publishing Group