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Differential expression and interaction specificity of heterotrimeric G-protein family in Brassica nigra reveal their developmental-and condition-specific roles

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Title Differential expression and interaction specificity of heterotrimeric G-protein family in Brassica nigra reveal their developmental-and condition-specific roles
 
Creator Kumar, Roshan
Arya, Gulab C.
Bisht, Naveen C.
 
Subject Brassica nigra
Gene expression
Heterotrimeric G-proteins
Polyploidy
Protein–protein interaction
Signal transduction
 
Description Accepted date: September 3, 2014
Heterotrimeric G-proteins, comprised of α, β and γ subunits, are important signal transducers across phyla. The G-proteins are well characterized in the model plants Arabidopsis and rice, and their inventories are possible from a few other plant species; however, information about the roles played by G-proteins in regulating various growth and developmental traits particularly from polyploid crops is still awaited. In this study, we have isolated one Gα (BniB.Gα1), three Gβ (BniB.Gβ1-BniB.Gβ3) and four Gγ (BniB.Gγ1-BniB.Gγ4) coding sequences from the paleopolyploid Brassica nigra, a major condiment crop of the Brassicaceae family. Sequence and phylogenetic analysis revealed that whole-genome triplication events in the Brassica lineage had proportionally increased the inventory of the Gβ subunit, but not of the Gα and Gγ subunits in B. nigra. Real-time quantitative reverse transcription-PCR (qRT-PCR) analysis showed that members of the G-protein subunit genes have distinct temporal and spatial expression patterns and were differentially altered in response to various stress and phytohormone treatments, thereby suggesting differential transcriptional regulation of G-protein genes in B. nigra. Interestingly, specific members of G-protein subunits were co-expressed across plant developmental stages, and in response to different elicitor treatments. Yeast-based interaction screens further predicted that the B. nigra G-protein subunits interacted in most of the possible combinations, although showing a high degree of interaction specificity between different G-protein subunits. Our data on physical interactions coupled with the co-expression pattern of the multiple G-protein subunit genes suggested that tissue- and condition-specific functional combinations of Gαβγ heterotrimers may exist in paleopolyploid B. nigra, to control diverse growth and development processes.
This work was supported by the National Institute of Plant
Genome Research (NIPGR), India [core grant to N.C.B.]; the
University Grant Commission (India) and Council for
Scientific and Industrial Research (India) [Junior Research
Fellowships to R.K. and G.C.A.].
 
Date 2015-12-17T08:58:06Z
2015-12-17T08:58:06Z
2014
 
Type Article
 
Identifier Plant and Cell Physiology, 55(11): 1954-1968
0032-0781
http://172.16.0.77:8080/jspui/handle/123456789/424
http://pcp.oxfordjournals.org/content/55/11/1954.long
10.1093/pcp/pcu126
 
Language en_US
 
Publisher Oxford University Press