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Virus-induced gene silencing is a versatile tool for unraveling the functional relevance of multiple abiotic-stress-responsive genes in crop plants

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Title Virus-induced gene silencing is a versatile tool for unraveling the functional relevance of multiple abiotic-stress-responsive genes in crop plants
 
Creator Ramegowda, Venkategowda
Mysore, Kirankumar S.
Senthil-Kumar, Muthappa
 
Subject abiotic stress
functional genomics of crop plants
plant viruses
post-transcriptional gene silencing
virus-induced gene silencing
 
Description Accepted date: 19 June 2014
Virus-induced gene silencing (VIGS) is an effective tool for gene function analysis in plants. Over the last decade, VIGS has been successfully used as both a forward and reverse genetics technique for gene function analysis in various model plants, as well as crop plants. With the increased identification of differentially expressed genes under various abiotic stresses through high-throughput transcript profiling, the application of VIGS is expected to be important in the future for functional characterization of a large number of genes. In the recent past, VIGS was proven to be an elegant tool for functional characterization of genes associated with abiotic stress responses. In this review, we provide an overview of how VIGS is used in different crop species to characterize genes associated with drought-, salt-, oxidative- and nutrient-deficiency-stresses. We describe the examples from studies where abiotic stress related genes are characterized using VIGS. In addition, we describe the major advantages of VIGS over other currently available functional genomics tools. We also summarize the recent improvements, limitations and future prospects of using VIGS as a tool for studying plant responses to abiotic stresses.
VIGS-based projects at Muthappa Senthil-Kumar’s laboratory are
supported by core funding from the National Institute of Plant
Genome Research and at Kirankumar S. Mysore’s laboratory
by The Samuel Roberts Noble Foundation. Authors thank Mr.
Mehanathan Muthamilarasan and Dr. Aiswarya Baruah for critical reading of the manuscript and Ms. Jackie Kelley for help with
editing the manuscript.
 
Date 2015-12-22T09:53:38Z
2015-12-22T09:53:38Z
2014
 
Type Article
 
Identifier Front. Plant Sc., 5: 323
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/444
http://journal.frontiersin.org/article/10.3389/fpls.2014.00323/abstract
10.3389/fpls.2014.00323
 
Language en_US
 
Publisher Frontiers Media S.A.