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Comprehensive expression analysis of rice Armadillo gene family during abiotic stress and development

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Title Comprehensive expression analysis of rice Armadillo gene family during abiotic stress and development
 
Creator Sharma, Manisha
Singh, Amarjeet
Shankar, Alka
Pandey, Amita
Baranwal, Vinay
Kapoor, Sanjay
Tyagi, Akhilesh K.
Pandey, Girdhar K.
 
Subject Armadillo
abiotic stress
development
signal transduction
 
Description Accepted date: 30 November 2013
Genes in the Armadillo (ARM)-repeat superfamily encode proteins with a range of developmental and physiological processes in unicellular and multicellular eukaryotes. These 42 amino acid, long tandem repeat-containing proteins have been abundantly recognized in many plant species. Previous studies have confirmed that Armadillo proteins constitute a multigene family in Arabidopsis. In this study, we performed a computational analysis in the rice genome (Oryza sativa L. subsp. japonica), and identified 158 genes of Armadillo superfamily. Phylogenetic study classified them into several arbitrary groups based on a varying number of non-conserved ARM repeats and accessory domain(s) associated with them. An in-depth analysis of gene expression through microarray and Q-PCR revealed a number of ARM proteins expressing differentially in abiotic stresses and developmental conditions, suggesting a potential roles of this superfamily in development and stress signalling. Comparative phylogenetic analysis between Arabidopsis and rice Armadillo genes revealed a high degree of evolutionary conservation between the orthologues in two plant species. The non-synonymous and synonymous substitutions per site ratios (Ka/Ks) of duplicated gene pairs indicate a purifying selection. This genome-wide identification and expression analysis provides a basis for further functional analysis of Armadillo genes under abiotic stress and reproductive developmental condition in the plant lineage.
This work is partially supported by internal grants of
University of Delhi and Department of Biotechnology
(DBT) India to GKP. M.S acknowledge University
Grants Commission (UGC), India for fellowship. AS, VB
and AlS acknowledge Council of Scientific Research
(CSIR) for their fellowship.
 
Date 2015-12-23T11:25:47Z
2015-12-23T11:25:47Z
2014
 
Type Article
 
Identifier DNA Res., 21(3): 267-283
1756-1663
http://172.16.0.77:8080/jspui/handle/123456789/456
http://dnaresearch.oxfordjournals.org/content/21/3/267
10.1093/dnares/dst056
 
Language en_US
 
Publisher Oxford University Press