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Over-expression of a rice tau class glutathione S-transferase gene improves tolerance to salinity and oxidative stresses in Arabidopsis

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Title Over-expression of a rice tau class glutathione S-transferase gene improves tolerance to salinity and oxidative stresses in Arabidopsis
 
Creator Sharma, Raghvendra
Sahoo, Annapurna
Devendran, Ragunathan
Jain, Mukesh
 
Subject rice tau class GST gene
Glutathione S-transferases (GSTs)
Arabidopsis
Oxidative Stresses
Salinity
rice
 
Description Accepted date: February 27, 2014
Glutathione S-transferases (GSTs) are multifunctional proteins encoded by large gene family in plants, which play important role in cellular detoxification of several endobiotic and xenobiotic compounds. Previously, we suggested the diverse roles of rice GST gene family members in plant development and various stress responses based on their differential expression. In this study, we report the functional characterization of a rice tau class GST gene, OsGSTU4. OsGSTU4 fusion protein was found to be localized in nucleus and cytoplasm. The over-expression of OsGSTU4 in E. coli resulted in better growth and higher GST activity under various stress conditions. Further, we raised over-expression transgenic Arabidopsis plants to reveal its in planta function. These transgenic lines showed reduced sensitivity towards plant hormones, auxin and abscisic acid. Various analyses revealed improved tolerance in transgenic Arabidopsis plants towards salinity and oxidative stresses, which may be attributed to the lower accumulation of reactive oxygen species and enhanced GST activity. In addition, microarray analysis revealed up-regulation of several genes involved in stress responses and cellular detoxification processes in the transgenic plants as compared to wild-type. These results suggest that OsGSTU4 can be used as a good candidate for the generation of stress-tolerant crop plants.
This work was financially supported by the Department of Biotechnology, Government of India, under Innovative Young Biotechnologists Award
scheme (grant numbers BT/BI/12/040/2005 and BT/02/IYBA/2010) and core grant from NIPGR. The funders had no role in study design, data collection and
analysis, decision to publish, or preparation of the manuscript.
 
Date 2015-12-23T11:37:19Z
2015-12-23T11:37:19Z
2014
 
Type Article
 
Identifier PLoS One, 9(3): e92900
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/457
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0092900
10.1371/journal.pone.0092900
 
Language en_US
 
Publisher PLOS