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Agroinfiltration by cytokinin-producing Agrobacterium sp. strain GV3101 primes defense responses in Nicotiana tabacum

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Title Agroinfiltration by cytokinin-producing Agrobacterium sp. strain GV3101 primes defense responses in Nicotiana tabacum
 
Creator Sheikh, Arsheed Hussain
Raghuram, Badmi
Eschen-Lippold, Lennart
Scheel, Dierk
Lee, Justin
Sinha, Alok Krishna
 
Subject Nicotiana tabacum
Agrobacterium
 
Description Accepted date: 14 July 2014
Transient infiltrations in tobacco are commonly used in plant studies, but the host response to different disarmed Agrobacterium strains is not fully understood. The present study shows that pretreatment with disarmed Agrobacterium tumefaciens GV3101 primes the defense response to subsequent infection by Pseudomonas syringae in Nicotiana tabacum. The presence of a trans-zeatin synthase (tzs) gene in strain GV3101 may be partly responsible for the priming response, as the tzs-deficient Agrobacterium sp. strain LBA4404 only weakly imparts such responses. Besides inducing the expression of defense-related genes like PR-1 and NHL10, GV3101 pretreatment increased the expression of tobacco mitogen-activated protein kinase (MAPK) pathway genes like MEK2, WIPK (wound-induced protein kinase), and SIPK (salicylic acid-induced protein kinase). Furthermore, the GV3101 strain showed a stronger effect than the LBA4404 strain in activating phosphorylation of the tobacco MAPK, WIPK and SIPK, which presumably prime the plant immune machinery. Lower doses of exogenously applied cytokinins increased the activation of MAPK, while higher doses decreased the activation, suggesting a balanced level of cytokinins is required to generate defense response in planta. The current study serves as a cautionary warning for plant researchers over the choice of Agrobacterium strains and their possible consequences on subsequent pathogen-related studies.
The authors thank T. Roitsch, University of Copenhagen, Denmark for
kindly providing P. syringae pv. tabaci and the 4xJERE:ipt construct; F.
Trempel for help with ROS assays. A. H. Sheikh acknowledges Council of
Scientific and Industrial Research, India, and Deutscher Akademischer
Austauschdienst, Germany, and B. Raghuram thanks the Department of
Biotechnology, Government of India for fellowship. This work was supported by the core grant of National Institute of Plant Genome Research,
Department of Biotechnology, Government of India to A. K. S. Sinha.
 
Date 2015-12-28T09:22:16Z
2015-12-28T09:22:16Z
2014
 
Type Article
 
Identifier Mol. Plant Microbe Interactions, 27(11): 1175-1185
0894-0282
http://172.16.0.77:8080/jspui/handle/123456789/459
http://apsjournals.apsnet.org/doi/abs/10.1094/MPMI-04-14-0114-R
10.1094/MPMI-04-14-0114-R
 
Language en_US
 
Publisher American Phytopathological Society