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Genome-wide expressional and functional analysis of calcium transport elements during abiotic stress and development in rice

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Title Genome-wide expressional and functional analysis of calcium transport elements during abiotic stress and development in rice
 
Creator Singh, Amarjeet
Kanwar, Poonam
Yadav, Akhilesh K.
Mishra, Manali
Jha, Saroj K.
Baranwal, Vinay
Pandey, Amita
Kapoor, Sanjay
Tyagi, Akhilesh K.
Pandey, Girdhar K.
 
Subject abiotic stress
Ca2+ transporter
development
expression
signal transduction
 
Description Accepted date: 21 November 2013
Ca²⁺ homeostasis is required to maintain a delicate balance of cytosolic Ca²⁺ during normal and adverse growth conditions. Various Ca²⁺ transporters actively participate to maintain this delicate balance especially during abiotic stresses and developmental events in plants. In this study, we present a genome-wide account, detailing expression profiles, subcellular localization and functional analysis of rice Ca²⁺ transport elements. Exhaustive in silico data mining and analysis resulted in the identification of 81 Ca²⁺ transport element genes, which belong to various groups such as Ca²⁺-ATPases (pumps), exchangers, channels, glutamate receptor homologs and annexins. Phylogenetic analysis revealed that different Ca²⁺ transporters are evolutionarily conserved across different plant species. Comprehensive expression analysis by gene chip microarray and quantitative RT-PCR revealed that a substantial proportion of Ca²⁺ transporter genes were expressed differentially under abiotic stresses (salt, cold and drought) and reproductive developmental stages (panicle and seed) in rice. These findings suggest a possible role of rice Ca²⁺ transporters in abiotic stress and development triggered signaling pathways. Subcellular localization of Ca²⁺ transporters from different groups in Nicotiana benthamiana revealed their variable localization to different compartments, which could be their possible sites of action. Complementation of Ca²⁺ transport activity of K616 yeast mutant by Ca²⁺-ATPase OsACA7 and involvement in salt tolerance verified its functional behavior. This study will encourage detailed characterization of potential candidate Ca²⁺ transporters for their functional role in planta.
This work was partially supported
by grants from the University of Delhi, Department of
Biotechnology, Department of Science and Technology, and the Council of Scientific and Industrial
Research (CSIR), India, to GKP. AS, PK, AKY and
VB acknowledge CSIR for their research fellowship.
 
Date 2015-12-29T06:36:32Z
2015-12-29T06:36:32Z
2014
 
Type Article
 
Identifier FEBS Journal, 281(3): 894-915
1742-4658
http://172.16.0.77:8080/jspui/handle/123456789/462
http://onlinelibrary.wiley.com/doi/10.1111/febs.12656/abstract
10.1111/febs.12656
 
Language en_US
 
Publisher John Wiley & Sons