Genome-wide expressional and functional analysis of calcium transport elements during abiotic stress and development in rice
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Title |
Genome-wide expressional and functional analysis of calcium transport elements during abiotic stress and development in rice
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Creator |
Singh, Amarjeet
Kanwar, Poonam Yadav, Akhilesh K. Mishra, Manali Jha, Saroj K. Baranwal, Vinay Pandey, Amita Kapoor, Sanjay Tyagi, Akhilesh K. Pandey, Girdhar K. |
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Subject |
abiotic stress
Ca2+ transporter development expression signal transduction |
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Description |
Accepted date: 21 November 2013
Ca²⁺ homeostasis is required to maintain a delicate balance of cytosolic Ca²⁺ during normal and adverse growth conditions. Various Ca²⁺ transporters actively participate to maintain this delicate balance especially during abiotic stresses and developmental events in plants. In this study, we present a genome-wide account, detailing expression profiles, subcellular localization and functional analysis of rice Ca²⁺ transport elements. Exhaustive in silico data mining and analysis resulted in the identification of 81 Ca²⁺ transport element genes, which belong to various groups such as Ca²⁺-ATPases (pumps), exchangers, channels, glutamate receptor homologs and annexins. Phylogenetic analysis revealed that different Ca²⁺ transporters are evolutionarily conserved across different plant species. Comprehensive expression analysis by gene chip microarray and quantitative RT-PCR revealed that a substantial proportion of Ca²⁺ transporter genes were expressed differentially under abiotic stresses (salt, cold and drought) and reproductive developmental stages (panicle and seed) in rice. These findings suggest a possible role of rice Ca²⁺ transporters in abiotic stress and development triggered signaling pathways. Subcellular localization of Ca²⁺ transporters from different groups in Nicotiana benthamiana revealed their variable localization to different compartments, which could be their possible sites of action. Complementation of Ca²⁺ transport activity of K616 yeast mutant by Ca²⁺-ATPase OsACA7 and involvement in salt tolerance verified its functional behavior. This study will encourage detailed characterization of potential candidate Ca²⁺ transporters for their functional role in planta. This work was partially supported by grants from the University of Delhi, Department of Biotechnology, Department of Science and Technology, and the Council of Scientific and Industrial Research (CSIR), India, to GKP. AS, PK, AKY and VB acknowledge CSIR for their research fellowship. |
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Date |
2015-12-29T06:36:32Z
2015-12-29T06:36:32Z 2014 |
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Type |
Article
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Identifier |
FEBS Journal, 281(3): 894-915
1742-4658 http://172.16.0.77:8080/jspui/handle/123456789/462 http://onlinelibrary.wiley.com/doi/10.1111/febs.12656/abstract 10.1111/febs.12656 |
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Language |
en_US
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Publisher |
John Wiley & Sons
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