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Balanced activity of microRNA166/165 and its target transcripts from the class III homeodomain leucine-zipper family regulates root growth in Arabidopsis thaliana
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View Archive Info| Field | Value | |
| Title |
Balanced activity of microRNA166/165 and its target transcripts from the class III homeodomain leucine-zipper family regulates root growth in Arabidopsis thaliana
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| Creator |
Singh, Archita
Singh, Sharmila Panigrahi, Kishore C. S. Reski, Ralf Sarkar, Ananda K. |
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| Subject |
miR166/165
microRNA RAM Root meristem Small RNA |
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| Description |
Accepted date: 16 January 2014
Overexpression of miR166/165 down-regulates target HD - ZIP IIIs and promotes root growth by enhancing cell division and meristematic activity, whereas overexpression of HD - ZIP IIIs inhibits root growth in Arabidopsis thaliana. Post-embryonic growth of higher plants is maintained by active meristems harbouring undifferentiated cells. Shoot and root apical meristems (SAM and RAM) utilize both similar and distinct signalling mechanisms for their maintenance in Arabidopsis thaliana. An important regulatory role in this context has the interaction of microRNAs with their target mRNAs, mostly encoding transcription factors. One class of microRNA166/165 (miR166/165) has been implicated in the maintenance of SAM and vascular patterning. Here, we show that miR166/165 plays an important role in root growth also by negatively regulating its target transcripts, HD-ZIP IIIs, in the RAM. While overexpression of miR166 promotes RAM activity, overexpression of its targets reduces RAM activity. These results reveal a conserved role of miR166/165 in the maintenance of SAM and RAM activity in A. thaliana. We thank DBT (Department of Biotechnology, Government of India) for a Ramalingaswami fellowship (BT/HRD/ 35/02/06/2008) grant to AKS, CSIR (Council for Scientific and Industrial Research, India) for a fellowship to AS and SS, and NIPGR (National Institute of Plant Genome Research, India) for internal funding and facility to carry out this work. We thank ABRC for Arabidopsis seeds and Prof. X. Wang (Institute of Genetics and Developmental Biology, China) for eTM-miR166. |
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| Date |
2015-12-29T07:13:08Z
2015-12-29T07:13:08Z 2014 |
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| Type |
Article
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| Identifier |
Plant Cell Reports, 33(6): 945-953
1432-203X http://172.16.0.77:8080/jspui/handle/123456789/463 http://link.springer.com/article/10.1007%2Fs00299-014-1573-z 10.1007/s00299-014-1573-z |
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| Language |
en_US
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| Publisher |
Springer
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