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OsAlba1, a dehydration-responsive nuclear protein of rice (Oryza sativa L. ssp. indica), participates in stress adaptation

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Title OsAlba1, a dehydration-responsive nuclear protein of rice (Oryza sativa L. ssp. indica), participates in stress adaptation
 
Creator Verma, Jitendra Kumar
Gayali, Saurabh
Dass, Suchismita
Kumar, Amit
Parveen, Shaista
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Alba family proteins
Dehydration response
Dimer modelling
DNA-binding proteins
Dual localization
Transcriptional regulation
 
Description Alba proteins have exhibited great functional plasticity through the course of evolution and constitute a superfamily that spans across three domains of life. Earlier, we had developed the dehydration-responsive nuclear proteome of an indica rice cultivar, screening of which led to the identification of an Alba protein. Here we describe, for the first time, the complete sequence of the candidate gene OsAlba1, its genomic organization, and possible function/s in plant. Phylogenetic analysis showed its close proximity to other monocots as compared to dicot Alba proteins. Protein-DNA interaction prediction indicates a DNA-binding property for OsAlba1. Confocal microscopy showed the localization of OsAlba1-GFP fusion protein to the nucleus, and also sparsely to the cytoplasm. Water-deficit conditions triggered OsAlba1 expression suggesting its function in dehydration stress, possibly through an ABA-dependent pathway. Functional complementation of the yeast mutant ΔPop6 established that OsAlba1 also functions in oxidative stress tolerance. The preferential expression of OsAlba1 in the flag leaves implies its role in grain filling. Our findings suggest that the Alba components such as OsAlba1, especially from a plant where there is no evidence for a major chromosomal role, might play important function in stress adaptation.
This work was supported by grants from the Council of Scientific and Industrial Research (CSIR), and the Department of Biotechnology (DBT), Govt. of India to N.C. The authors thank the DBT for
providing financial assistance to J.K.V., A.K., and S.P., and the CSIR
for providing fellowship to S.G. S.D. has received fellowship from
the National Institute of Plant Genome Research (NIPGR), New Delhi. The authors also thank Mr. Jasbeer Singh for illustration and
graphical representation.
 
Date 2015-12-29T11:18:57Z
2015-12-29T11:18:57Z
2014
 
Type Article
 
Identifier Phytochemistry, 100: 16-25
0031-9422
http://172.16.0.77:8080/jspui/handle/123456789/472
http://www.sciencedirect.com/science/article/pii/S0031942214000399
10.1016/j.phytochem.2014.01.015
 
Language en_US
 
Publisher Elsevier B.V.