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Transcriptome analysis of Catharanthus roseus for gene discovery and expression profiling

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Title Transcriptome analysis of Catharanthus roseus for gene discovery and expression profiling
 
Creator Verma, Mohit
Ghangal, Rajesh
Sharma, Raghvendra
Sinha, Alok Krishna
Jain, Mukesh
 
Subject Catharanthus roseus
Chickpea
Expression Profiling
Transcriptome Analysis
Gene Discovery
 
Description Accepted date: June 28, 2014
The medicinal plant, Catharanthus roseus, accumulates wide range of terpenoid indole alkaloids, which are well documented therapeutic agents. In this study, deep transcriptome sequencing of C. roseus was carried out to identify the pathways and enzymes (genes) involved in biosynthesis of these compounds. About 343 million reads were generated from different tissues (leaf, flower and root) of C. roseus using Illumina platform. Optimization of de novo assembly involving a two-step process resulted in a total of 59,220 unique transcripts with an average length of 1284 bp. Comprehensive functional annotation and gene ontology (GO) analysis revealed the representation of many genes involved in different biological processes and molecular functions. In total, 65% of C. roseus transcripts showed homology with sequences available in various public repositories, while remaining 35% unigenes may be considered as C. roseus specific. In silico analysis revealed presence of 11,620 genic simple sequence repeats (excluding mono-nucleotide repeats) and 1820 transcription factor encoding genes in C. roseus transcriptome. Expression analysis showed roots and leaves to be actively participating in bisindole alkaloid production with clear indication that enzymes involved in pathway of vindoline and vinblastine biosynthesis are restricted to aerial tissues. Such large-scale transcriptome study provides a rich source for understanding plant-specialized metabolism, and is expected to promote research towards production of plant-derived pharmaceuticals.
This work was financially supported by the core grant of the National Institute of Plant Genome Research. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
 
Date 2015-12-29T11:27:01Z
2015-12-29T11:27:01Z
2014
 
Type Article
 
Identifier PLoS One, 9(7): e103583
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/473
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0103583
10.1371/journal.pone.0103583
 
Language en_US
 
Publisher PLOS