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Exploring genetic variability within lentil (Lens culinaris Medik.) and across related legumes using a newly developed set of microsatellite markers

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Title Exploring genetic variability within lentil (Lens culinaris Medik.) and across related legumes using a newly developed set of microsatellite markers
 
Creator Verma, Priyanka
Sharma, Tilak R.
Srivastava, Prem S.
Abdin, M. Z.
Bhatia, Sabhyata
 
Subject Microsatellites
SSRs
Lentil
Enriched library
Genetic diversity
Cross-genera transferability
 
Description Accepted date: 21 May 2014
Lentil (Lens culinaris Medik.) is an economically important grain legume, yet the genetic and genomic resources remain largely uncharacterized and unexploited in this crop. Microsatellites have become markers of choice for crop improvement applications. Hence, simple sequence repeat (SSR) markers were developed for lentil through the construction of genomic library enriched for GA/CT motifs. As a result 122 functional SSR primer pairs were developed from 151 microsatellite loci and validated in L. culinaris cv. Precoz. Thirty three SSR markers were utilized for the analysis of genetic relationships between cultivated and wild species of Lens and related legumes. A total of 123 alleles were amplified at 33 loci ranging from 2-5 alleles with an average of 3.73 alleles per locus. Polymorphic information content (PIC) for all the loci ranged from 0.13 to 0.99 with an average of 0.66 per locus. Varied levels of cross genera transferability were obtained ranging from 69.70 % across Pisum sativum to 12.12 % across Vigna radiata. The UPGMA based dendrogram was able to establish the uniqueness of each genotype and grouped them into two major clusters clearly resolving the genetic relationships within lentil and related species. The new set of SSR markers reported here were efficient and highly polymorphic and would add to the existing repertoire of lentil SSR markers to be utilized in molecular breeding. Moreover, the improved knowledge about intra- and inter-specific genetic relationships would facilitate germplasm utilization for lentil improvement.
We are grateful to Dr. T.R. Sharma (CSK, Himachal Pradesh University, Palampur) for providing the accessions of
lentil. We are grateful to the International Crops Research Institute for
the Semi-Arid Tropics (ICRISAT) India, National Bureau for Plant
Genetic Resources (NBPGR) India, Maharana Pratap Agriculture
University (MPAU) India, and Australian Medicago Genetic
Resource Centre, SARDI, Australia for providing us the seed material
of different accessions of legumes. This research work was supported
by the National Institute of Plant Genome Research (NIPGR), New
Delhi, India. Financial assistance was supported by The Department
of Biotechnology (DBT), Government of India, under the Grant No.
BT/01/08/NRC-PBI/01.
 
Date 2015-12-29T11:34:48Z
2015-12-29T11:34:48Z
2014
 
Type Article
 
Identifier Mol. Biol. Rep., 41(9): 5607-5625
1573-4978
http://172.16.0.77:8080/jspui/handle/123456789/474
http://link.springer.com/article/10.1007%2Fs11033-014-3431-z
10.1007/s11033-014-3431-z
 
Language en_US
 
Publisher Springer