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Development of novel microRNA-based genetic markers in foxtail millet for genotyping applications in related grass species

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Title Development of novel microRNA-based genetic markers in foxtail millet for genotyping applications in related grass species
 
Creator Yadav, Chandra Bhan
Muthamilarasan, Mehanathan
Pandey, Garima
Khan, Yusuf
Prasad, Manoj
 
Subject Foxtail millet (Setaria italica L.)
microRNA-based markers
Cross-transferability
Phylogenetics
Millets
Cereals
 
Description Accepted date: 17 June 2014
DNA markers are important in molecular breeding, and, hence, considering its prominence, a variety of DNA-based molecular markers have been explored and developed for expediting crop improvement programs. microRNA (miRNA)-based molecular marker is a type of functional markers exploited predominantly in animal sciences, but reported in very few plants. Considering the efficacy, stability and transferability potential of the miRNA-based markers, the present study was conducted to develop these markers in the model crop foxtail millet. The pre-miRNA sequences of foxtail millet and other related grasses including rice, maize, wheat, sorghum and Brachypodium were retrieved and aligned for identifying the conserved regions. One hundred and seventy-six primer pairs were designed for these consensus sequences, and all these 176 miRNA-based markers were mapped onto foxtail millet genome. Of the 176 markers, 66 were chosen for further experimentations based on representing the nine chromosomes of foxtail millet and presence of highly conserved regions. All the 66 markers showed 100 % amplification in five cultivars of foxtail millet. Moreover, all the markers showed a higher level of cross-genera transferability potential with an average of ~67 % in millets and non-millet species. This is the first report on the development of novel miRNA-based markers in foxtail millet. Promisingly, these markers would serve as novel genotyping tool for various molecular breeding approaches aiming at crop improvement in millets and non-millet species.
The authors’ work in this area was
supported by the core grant of NIPGR. Mr. Mehanathan
Muthamilarasan and Ms. Garima Pandey acknowledge the
award of Research Fellowships from University Grants
Commission, New Delhi, India.
 
Date 2015-12-29T11:51:49Z
2015-12-29T11:51:49Z
2014
 
Type Article
 
Identifier Mol. Breed., 34(4): 2219-2224
1572-9788
http://172.16.0.77:8080/jspui/handle/123456789/475
http://link.springer.com/article/10.1007%2Fs11032-014-0137-9
10.1007/s11032-014-0137-9
 
Language en_US
 
Publisher Springer