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Biofortification of oilseed Brassica juncea with the anti-cancer compound glucoraphanin by suppressing GSL-ALK gene family

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Title Biofortification of oilseed Brassica juncea with the anti-cancer compound glucoraphanin by suppressing GSL-ALK gene family
 
Creator Augustine, Rehna
Bisht, Naveen C.
 
Subject Metabolic engineering
Plant biotechnology
 
Description Accepted date: 10 November 2015
Glucosinolates are amino acids derived secondary metabolites, invariably present in Brassicales, which have huge health and agricultural benefits. Sulphoraphane, the breakdown product of glucosinolate glucoraphanin is known to posses anti-cancer properties. AOP (2-oxoglutarate-dependent dioxygenases) or GSL-ALK enzyme catalyzes the conversion of desirable glucoraphanin to deleterious gluconapin and progoitrin, which are present in very high amounts in most of the cultivable Brassica species including Brassica juncea. In this study we showed that B. juncea encodes four functional homologs of GSL-ALK gene and constitutive silencing of GSL-ALK homologs resulted in accumulation of glucoraphanin up to 43.11 μmoles g(-1) DW in the seeds with a concomitant reduction in the anti-nutritional glucosinolates. Glucoraphanin content was found remarkably high in leaves as well as sprouts of the transgenic lines. Transcript quantification of high glucoraphanin lines confirmed significant down-regulation of GSL-ALK homologs. Growth and other seed quality parameters of the transgenic lines did not show drastic difference, compared to the untransformed control. High glucoraphanin lines also showed higher resistance towards stem rot pathogen Sclerotinia sclerotiorum. Our results suggest that metabolic engineering of GSL-ALK has huge potential for enriching glucoraphanin content, and improve the oil quality and vegetable value of Brassica crops.
The work was supported by IYBA-2012 grant (BT/06/IYBA/2012) of Department of Biotechnology India to NCB.
RA was funded with short term research fellowship from NIPGR, India. We are thankful to Dr. Prabodh K. Bajpai
for his assistance in statistical analysis using SPSS software and Dr. Pritam Kalia (Division of Vegetable Science,
IARI, New Delhi, India) for providing B. oleracea seeds. We are grateful to central instrumentation facility and
plant growth facility at NIPGR. Technical assistance from Vinod Kumar is also acknowledged.
 
Date 2015-12-30T06:28:58Z
2015-12-30T06:28:58Z
2015
 
Type Article
 
Identifier Scientific Reports, 5: 18005
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/478
http://www.nature.com/articles/srep18005
10.1038/srep18005
 
Language en_US
 
Publisher Nature Publishing Group