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Biotic elicitors and mechanical damage modulate glucosinolate accumulation by co-ordinated interplay of glucosinolate biosynthesis regulators in polyploid Brassica juncea

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Title Biotic elicitors and mechanical damage modulate glucosinolate accumulation by co-ordinated interplay of glucosinolate biosynthesis regulators in polyploid Brassica juncea
 
Creator Augustine, Rehna
Bisht, Naveen C.
 
Subject Biotic elicitors
Brassica juncea
Glucose
Glucosinolates
MYB genes
Plant defense
Wounding
 
Description Accepted date: 26 May 2015
Glucosinolates are nitrogen and sulfur containing secondary metabolites found mainly in the Brassicaceae. They function as plant defense compounds against a broad spectrum of pathogens and pests. Since these molecules form part of the plant defense mechanism, glucosinolate biosynthesis may be modulated by environmental signals leading to activation of a biological stress response. In the current study, we have mimicked such conditions by exogenously applying biotic elicitors such as methyl jasmonate, salicylic acid, glucose and mechanical injury in Brassica juncea seedling over a time course experiment. We found that total glucosinolates over-accumulated under these stress conditions with maximum accumulation observed 24h post treatment. Indole glucosinolates like 1-methoxy-indol-3-ylmethyl and its precursor indol-3-methyl glucosinolates showed a more significant induction compared to aliphatic glucosinolates thereby suggesting a prominent role of indole glucosinolates during plant defense response in B. juncea seedlings. In contrast, the higher amounts of aliphatic glucosinolates were less regulated by the tested biotic elicitors in B. juncea. Expression profiling of multiple homologs of key transcriptional regulators of glucosinolate biosynthesis further showed that a complex interplay of these regulators exists in polyploid B. juncea where they exert co-ordinated and overlapping effects toward altering glucosinolate accumulation. This study has a significant role toward understanding and augmenting plant defense mechanisms in B. juncea, a globally important oilseed crop of genus Brassica.
The work was supported by Department of Biotechnology
(India)-IYBA (2012) and the core-Grant from National Institute of
Plant Genome Research (NIPGR) to NCB. The short term NIPGR
research associate fellowship to RA is acknowledged. We thank
Dr. Michael Reichelt, Max Plank Institute of Chemical Ecology,
Jena, Germany for kindly providing glucosinolates standards and
Mr. Vinod Kumar for his technical assistance during glucosinolate
extraction. We acknowledge Dr. Arun Jagannath, Department of
Botany, University of Delhi (India) for critical evaluation and language editing of the revised manuscript. The central instrumentation facility and plant growth facility at NIPGR are acknowledged.
Two anonymous reviewers are also acknowledged, which helped
us to improve the presentation of manuscript.
 
Date 2015-12-30T08:48:19Z
2015-12-30T08:48:19Z
2015
 
Type Article
 
Identifier Phytochemistry, 117: 43-50
0031-9422
http://172.16.0.77:8080/jspui/handle/123456789/480
http://www.sciencedirect.com/science/article/pii/S0031942215300030
10.1016/j.phytochem.2015.05.015
 
Language en_US
 
Publisher Elsevier B.V.