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Stage-specific reprogramming of gene expression characterizes Lr48-mediated adult plant leaf rust resistance in wheat

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Title Stage-specific reprogramming of gene expression characterizes Lr48-mediated adult plant leaf rust resistance in wheat
 
Creator Dhariwal, Raman
Gahlaut, Vijay
Govindraj, Bhaganagare R.
Singh, Dharmendra
Mathur, Saloni
Vyas, Shailendra
Bandopadhyay, Rajib
Khurana, Jitendra Paul
Tyagi, Akhilesh K.
Prabhu, Kumble Vinod
Mukhopadhyay, Kunal
Balyan, Harindra Singh
Gupta, Pushpendra Kumar
 
Subject Triticum aestivum
Puccinia triticina
TDFs
Incompatible interaction
Compatible interaction
Recessive APR gene
 
Description Accepted date: 17 November 2014
Wheat genotype CSP44 carrying a recessive gene Lr48 exhibits adult plant resistance (APR; incompatible reaction) but gives a compatible reaction (susceptibility) at the seedling stage against leaf rust. A comparative gene expression analysis involving cDNA-amplified fragment length polymorphism (cDNA-AFLP) and quantitative PCR (qPCR) was carried out for incompatible and compatible reactions in the genotype CSP44. cDNA-AFLP analysis was conducted using RNA samples that were isolated from flag leaves following inoculation with leaf rust race 77-5 (the most virulent race) and also after mock inoculation. As many as 298 of a total of 493 expressed transcript-derived fragments (TDFs) exhibited differential expression (262 upregulated and 36 downregulated). Of these 298 TDFs, 48 TDFs were eluted from gels, re-amplified, cloned, and sequenced. Forty two of these 48 TDFs had homology with known genes involved in the following biological processes: energy production, metabolism, transport, signaling, defense response, plant-pathogen interaction, transcriptional regulation, translation, and proteolysis. The functions of the remaining six TDFs could not be determined; apparently, these represented some novel genes. The qPCR analysis for 18 TDFs (with known and unknown functions, but showing major differences in expression) was conducted using RNA isolated from the seedlings as well as from the adult plants. The expression of at least 11 TDFs was induced and that of 4 other TDFs attenuated or remained near normal in adult plants following leaf rust inoculations. The remaining three TDFs had non-specific/developmental stage-specific expression. Functional annotation of TDFs that were upregulated suggest that the APR was supported by transient recruitment and reprogramming of processes like perception and recognition of pathogen effector by receptors, followed by CDPK and MAPK signaling, transport, metabolism, and energy release.
PKG was awarded INSA Honorary Scientist and
NASI Senior Scientist Fellowships during the tenure of this study. Thanks
are due to the Department of Biotechnology, Government of India (grant
no. BT/PR6037/AGR/02/308/05), for providing financial support to carry
out this study. The authors are grateful to the Head, Regional Station,
Directorate of Wheat Research, Flowerdale, Shimla, for providing inoculum of the leaf rust pathogen.
 
Date 2016-01-04T05:50:32Z
2016-01-04T05:50:32Z
2015
 
Type Article
 
Identifier Funct. Integr. Genomics, 15(2): 233-245
1438-7948
http://172.16.0.77:8080/jspui/handle/123456789/499
http://link.springer.com/article/10.1007%2Fs10142-014-0416-x
10.1007/s10142-014-0416-x
 
Language en_US
 
Publisher Springer