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Genome-scale transcriptomic insights into molecular aspects of abiotic stress responses in chickpea

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Title Genome-scale transcriptomic insights into molecular aspects of abiotic stress responses in chickpea
 
Creator Garg, Rohini
Bhattacharjee, Annapurna
Jain, Mukesh
 
Subject Chickpea
Abiotic stress
Deep sequencing
Transcription factors
Metabolic pathways
 
Description Chickpea is an important legume crop plant and various abiotic stresses are the major constraints affecting its overall productivity. For discovery of candidate genes involved in abiotic stress responses, we employed RNA sequencing for transcriptome profiling of roots and shoots of chickpea seedlings subjected to desiccation, salinity, and cold stresses. In total, we generated more than 250 million high-quality reads from non-stressed and stressed tissue samples. Data analyses provided a comprehensive view of the dynamic transcriptional response of chickpea tissues to different abiotic stresses. Differential expression analysis identified a total of 11,640 chickpea transcripts showing response to at least one of the stress conditions. The reference-based transcriptome assembly was generated and at least 3,536 previously unannotated gene loci differentially expressed under abiotic stress conditions were identified. We observed extensive transcriptional reprogramming of genes involved in transcription regulation, energy metabolism, photosynthesis, hormonal responses, secondary metabolite biosynthesis and osmoprotectant metabolism under stress conditions. In addition, genes involved in post-translational modifications, RNA metabolic processes, and epigenetic regulation were also significantly highlighted. The comprehensive transcriptome analyses presented in this study revealed several potential key regulators of plant response to abiotic stresses and open avenues to carry out functional and applied genomic studies for improving abiotic stress tolerance in chickpea.
This work was financially supported by the Department of Science and Technology, Government of India, New Delhi,
under the INSPIRE Faculty Award scheme to RG and Australia-India
Strategic Research Fund (AISRF) scheme to MJ. AB acknowledges the
award of research fellowship from the Council of Scientific and Industrial Research, New Delhi. We are thankful to R. Kumari for help in
some data analysis.
 
Date 2016-01-04T06:12:01Z
2016-01-04T06:12:01Z
2015
 
Type Article
 
Identifier Plant Mol. Biol. Rep., 33(3): 388-400
1572-9818
http://172.16.0.77:8080/jspui/handle/123456789/501
http://link.springer.com/article/10.1007%2Fs11105-014-0753-x
10.1007/s11105-014-0753-x
 
Language en_US
 
Publisher Springer