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Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height

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Title Development of gene-based markers for use in construction of the chickpea (Cicer arietinum L.) genetic linkage map and identification of QTLs associated with seed weight and plant height
 
Creator Gupta, Shefali
Kumar, Tapan
Verma, Subodh
Bharadwaj, Chellapilla
Bhatia, Sabhyata
 
Subject Cicer arietinum
EST-based markers
Intra- specific linkage map
QTLs
Seed weight
 
Description Accepted date: 3 October 2015
Seed weight and plant height are important agronomic traits and contribute to seed yield. The objective of this study was to identify QTLs underlying these traits using an intra-specific mapping population of chickpea. A F11 population of 177 recombinant inbred lines derived from a cross between SBD377 (100-seed weight- 48 g and plant height- 53 cm) and BGD112 (100-seed weight- 15 g and plant height- 65 cm) was used. A total of 367 novel EST-derived functional markers were developed which included 187 EST-SSRs, 130 potential intron polymorphisms (PIPs) and 50 expressed sequence tag polymorphisms (ESTPs). Along with these, 590 previously published markers including 385 EST-based markers and 205 genomic SSRs were utilized. Of the 957 markers tested for analysis of parental polymorphism between the two parents of the mapping population, 135 (14.64 %) were found to be polymorphic. Of these, 131 polymorphic markers could be mapped to the 8 linkage groups. The linkage map had a total length of 1140.54 cM with an average marker density of 8.7 cM. The map was further used for QTL identification using composite interval mapping method (CIM). Two QTLs each for seed weight, qSW-1 and qSW-2 (explaining 11.54 and 19.24 % of phenotypic variance, respectively) and plant height, qPH-1 and qPH-2 (explaining 13.98 and 12.17 % of phenotypic variance, respectively) were detected. The novel set of genic markers, the intra-specific linkage map and the QTLs identified in the present study will serve as valuable genomic resources in improving the chickpea seed yield using marker-assisted selection (MAS) strategies.
This work was supported by the National
Institute of Plant Genome Research (NIPGR), New Delhi, India, Indian Agricultural Research Institute (IARI), New Delhi, India and
Department of Biotechnology (DBT), Government of India under
project number BT/01/08/NRC-PBI/01. The fellowships provided to
SG by Council for Scientific and Industrial Research (CSIR), India
and to SV by DBT, India is gratefully acknowledged.
 
Date 2016-01-05T06:43:16Z
2016-01-05T06:43:16Z
2015
 
Type Article
 
Identifier Mol. Biol. Rep., 42(11): 1571-1580
1573-4978
http://172.16.0.77:8080/jspui/handle/123456789/510
http://link.springer.com/article/10.1007%2Fs11033-015-3925-3
10.1007/s11033-015-3925-3
 
Language en_US
 
Publisher Springer