Record Details

Secretome analysis of chickpea reveals dynamic extracellular remodeling and identifies a Bet v1- like protein, CaRRP1 that participates in stress response

NIPGR Digital Knowledge Repository (NDKR)

View Archive Info
 
 
Field Value
 
Title Secretome analysis of chickpea reveals dynamic extracellular remodeling and identifies a Bet v1- like protein, CaRRP1 that participates in stress response
 
Creator Gupta, Sonika
Wardhan, Vijay
Kumar, Amit
Rathi, Divya
Pandey, Aarti
Chakraborty, Subhra
Chakraborty, Niranjan
 
Subject Plant molecular biology
Plant stress responses
 
Description Accepted date: 16 November 2015
Secreted proteins maintain cell structure and biogenesis besides acting in signaling events crucial for cellular homeostasis during stress adaptation. To understand the underlying mechanism of stress-responsive secretion, the dehydration-responsive secretome was developed from suspension-cultured cells of chickpea. Cell viability of the suspension culture remained unaltered until 96 h, which gradually declined at later stages of dehydration. Proteomic analysis led to the identification of 215 differentially regulated proteins, involved in a variety of cellular functions that include metabolism, cell defence, and signal transduction suggesting their concerted role in stress adaptation. One-third of the secreted proteins were devoid of N-terminal secretion signals suggesting a non-classical secretory route. Screening of the secretome identified a leaderless Bet v 1-like protein, designated CaRRP1, the export of which was inhibited by brefeldin A. We investigated the gene structure and genomic organization and demonstrated that CaRRP1 may be involved in stress response. Its expression was positively associated with abiotic and biotic stresses. CaRRP1 could complement the aberrant growth phenotype of yeast mutant, deficient in vesicular transport, indicating a partial overlap of protein secretion and stress response. Our study provides the most comprehensive analysis of dehydration-responsive secretome and the complex metabolic network operating in plant extracellular space.
This work was supported by grants (BT/PR12919/AGR/02/676 and BT/PR4016/AGR/16/327) from the Department
of Biotechnology (DBT), Govt. of India. S.G., V.W. and D.R. were supported by the predoctoral fellowship from
the Council of Scientific and Industrial Research (CSIR), Govt. of India and A.P. received postdoctoral fellowship
from the National Institute of Plant Genome Research, New Delhi. Dr. Suchismita Dass is thanked for assistance
with writing the manuscript. We thank Mr. Shankar Acharya for technical assistance and Mr. Jasbeer Singh for
illustrations and graphical representation in the manuscript.
 
Date 2016-01-05T07:23:02Z
2016-01-05T07:23:02Z
2015
 
Type Article
 
Identifier Scientific Reports, 5: 18427
2045-2322
http://172.16.0.77:8080/jspui/handle/123456789/512
http://www.nature.com/articles/srep18427
10.1038/srep18427
 
Language en_US
 
Publisher Nature Publishing Group