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Overexpression of an AP2/ERF type transcription factor OsEREBP1 confers biotic and abiotic stress tolerance in rice

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Title Overexpression of an AP2/ERF type transcription factor OsEREBP1 confers biotic and abiotic stress tolerance in rice
 
Creator Jisha, V.
Dampanaboina, Lavanya
Vadassery, Jyothilakshmi
Mithöfer, Axel
Kappara, Saivishnupriya
Ramanan, Rajeshwari
 
Subject Rice
Abiotic stress Tolerance
Biotic stress Tolerance
AP2/ERF
OsEREBP1
 
Description Accepted date: April 21, 2015
AP2/ERF-type transcription factors regulate important functions of plant growth and development as well as responses to environmental stimuli. A rice AP2/ERF transcription factor, OsEREBP1 is a downstream component of a signal transduction pathway in a specific interaction between rice (Oryza sativa) and its bacterial pathogen, Xoo (Xanthomonas oryzae pv. oryzae). Constitutive expression of OsEREBP1 in rice driven by maize ubiquitin promoter did not affect normal plant growth. Microarray analysis revealed that over expression of OsEREBP1 caused increased expression of lipid metabolism related genes such as lipase and chloroplastic lipoxygenase as well as several genes related to jasmonate and abscisic acid biosynthesis. PR genes, transcription regulators and Aldhs (alcohol dehydrogenases) implicated in abiotic stress and submergence tolerance were also upregulated in transgenic plants. Transgenic plants showed increase in endogenous levels of α-linolenate, several jasmonate derivatives and abscisic acid but not salicylic acid. Soluble modified GFP (SmGFP)-tagged OsEREBP1 was localized to plastid nucleoids. Comparative analysis of non-transgenic and OsEREBP1 overexpressing genotypes revealed that OsEREBP1 attenuates disease caused by Xoo and confers drought and submergence tolerance in transgenic rice. Our results suggest that constitutive expression of OsEREBP1 activates the jasmonate and abscisic acid signalling pathways thereby priming the rice plants for enhanced survival under abiotic or biotic stress conditions. OsEREBP1 is thus, a good candidate gene for engineering plants for multiple stress tolerance.
The work was supported by Department of Science and Technology (DST No: SR/SO/PS-68/2008) and Council for Scientific and Industrial Research (CSIR No: 9/1/BS/CCMB (4)/2012-13-PPD), Government of India.
 
Date 2016-01-06T05:16:32Z
2016-01-06T05:16:32Z
2015
 
Type Article
 
Identifier PLoS One, 10(6): e0127831
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/521
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127831
10.1371/journal.pone.0127831
 
Language en_US
 
Publisher PLOS