Differentially expressed seed aging responsive heat shock protein OsHSP18.2 implicates in seed vigor, longevity and improves germination and seedling establishment under abiotic stress
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Title |
Differentially expressed seed aging responsive heat shock protein OsHSP18.2 implicates in seed vigor, longevity and improves germination and seedling establishment under abiotic stress
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Creator |
Kaur, Harmeet
Petla, Bhanu P. Kamble, Nitin U. Singh, Ajeet Rao, Venkateswara Salvi, Prafull Ghosh, Shraboni Majee, Manoj |
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Subject |
sHSP
chaperone seed vigor CDT stress |
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Description |
Accepted date: 25 August 2015
Small heat shock proteins (sHSPs) are a diverse group of proteins and are highly abundant in plant species. Although majority of these sHSPs were shown to express specifically in seed, their potential function in seed physiology remains to be fully explored. Our proteomic analysis revealed that OsHSP18.2, a class II cytosolic HSP is an aging responsive protein as its abundance significantly increased after artificial aging in rice seeds. OsHSP18.2 transcript was found to markedly increase at the late maturation stage being highly abundant in dry seeds and sharply decreased after germination. Our biochemical study clearly demonstrated that OsHSP18.2 forms homooligomeric complex and is dodecameric in nature and functions as a molecular chaperone. OsHSP18.2 displayed chaperone activity as it was effective in preventing thermal inactivation of Citrate Synthase. Further, to analyze the function of this protein in seed physiology, seed specific Arabidopsis overexpression lines for OsHSP18.2 were generated. Our subsequent functional analysis clearly demonstrated that OsHSP18.2 has ability to improve seed vigor and longevity by reducing deleterious ROS accumulation in seeds. In addition, transformed Arabidopsis seeds also displayed better performance in germination and cotyledon emergence under adverse conditions. Collectively, our work demonstrates that OsHSP18.2 is an aging responsive protein which functions as a molecular chaperone and possibly protect and stabilize the cellular proteins from irreversible damage particularly during maturation drying, desiccation and aging in seeds by restricting ROS accumulation and thereby improves seed vigor, longevity and seedling establishment. This work was supported by the institute core grant from NIPGR, Department of Biotechnology, Government of India. |
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Date |
2016-01-06T09:45:41Z
2016-01-06T09:45:41Z 2015 |
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Type |
Article
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Identifier |
Front. Plant Sc., 6: 713
1664-462X http://172.16.0.77:8080/jspui/handle/123456789/525 http://journal.frontiersin.org/article/10.3389/fpls.2015.00713/abstract 10.3389/fpls.2015.00713 |
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Language |
en_US
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Publisher |
Frontiers Media S.A.
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