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Systemic cytosolic Ca2+ elevation is activated upon wounding and herbivory in Arabidopsis

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Title Systemic cytosolic Ca2+ elevation is activated upon wounding and herbivory in Arabidopsis
 
Creator Kiep, Victoria
Vadassery, Jyothilakshmi
Lattke, Justus
Maaß, Jan-Peter
Boland, Wilhelm
Peiter, Edgar
Mithöfer, Axel
 
Subject aequorin
Arabidopsis thaliana
calcium
herbivory
plant defence
systemic signalling
Two Pore Channel 1 (TPC1)
wounding
 
Description Calcium ion (Ca2+) signalling triggered by insect herbivory is an intricate network with multiple components, involving positive and negative regulators. Real-time, noninvasive imaging of entire Arabidopsis thaliana rosettes was employed to monitor cytosolic free calcium ([Ca2+]cyt) elevations in local and systemic leaves in response to wounding and Spodoptera littoralis feeding. Luminescence emitted by the cytosol-localized Ca2+ reporter aequorin was imaged using a high-resolution photon-counting camera system.
Spodoptera littoralis feeding on Arabidopsis induced both local and systemic [Ca2+]cyt elevations. Systemic [Ca2+]cyt signals were found predominantly in adjacent leaves with direct vascular connections to the treated leaf and appeared with a delay of 1 to 2 min. Simulated herbivory by wounding always induced a local [Ca2+]cyt response, but a systemic one only when the midrib was wounded. This systemic [Ca2+]cyt response was suppressed by the presence of insect-derived oral secretions as well as in a mutant of the vacuolar cation channel, Two Pore Channel 1 (TPC1).
Our results provide evidence that in Arabidopsis insect herbivory induces both local and systemic [Ca2+]cyt signals that distribute within the vascular system. The systemic [Ca2+]cyt signal could play an important signalling role in systemic plant defence.
The authors thank A. Lehr and P. Sharma (Max Planck
Institute for Chemical Ecology, MPI-CE) for excellent technical
assistance, M. Knight (University of Durham, UK) for providing the Apoaequorin-transformed Arabidopsis line, the
Plant Protection Centre of Bayer AG (Mannheim, Germany)
for providing Spodoptera littoralis egg clutches, A. Berg for
culturing caterpillars, and K. Peter (Martin Luther University,
MLU), T. Kr€gel (MPI-CE), A. Weber (MPI-CE), and the
u
MPI-CE glasshouse team for plant cultivation. This work was
supported by the Max Planck Society, the Ministry of
Agriculture and the Environment of the Federal State of
Sachsen-Anhalt, and the Deutsche Forschungsgemeinschaft
(DFG PE 1500/4-1).
 
Date 2016-01-06T10:11:01Z
2016-01-06T10:11:01Z
2015
 
Type Article
 
Identifier New Phytol., 207(4): 996-1004
1469-8137
http://172.16.0.77:8080/jspui/handle/123456789/527
http://onlinelibrary.wiley.com/wol1/doi/10.1111/nph.13493/abstract
10.1111/nph.13493
 
Language en_US
 
Publisher John Wiley & Sons