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The CRISPR-Cas system for plant genome editing: advances and opportunities

NIPGR Digital Knowledge Repository (NDKR)

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Title The CRISPR-Cas system for plant genome editing: advances and opportunities
 
Creator Kumar, Vinay
Jain, Mukesh
 
Subject CRISPR
Cas9
genome editing
mutation
protospacer adjacent motif
sgRNA
 
Description Accepted date: 23 September 2014
Genome editing is an approach in which a specific target DNA sequence of the genome is altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid enzymes, zinc-finger nucleases (ZFNs), and transcription activator-like effector nucleases (TALENs), and the CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated protein) system are being used for genome editing in various organisms including plants. The CRISPR–Cas system has been developed most recently and seems to be more efficient and less time-consuming compared with ZFNs or TALENs. This system employs an RNA-guided nuclease, Cas9, to induce double-strand breaks. The Cas9-mediated breaks are repaired by cellular DNA repair mechanisms and mediate gene/genome modifications. Here, we provide a detailed overview of the CRISPR–Cas system and its adoption in different organisms, especially plants, for various applications. Important considerations and future opportunities for deployment of the CRISPR–Cas system in plants for numerous applications are also discussed. Recent investigations have revealed the implications of the CRISPR–Cas system as a promising tool for targeted genetic modifications in plants. This technology is likely to be more commonly adopted in plant functional genomics studies and crop improvement in the near future.
The authors are grateful to the National Institute of Plant Genome Research
and the Department of Biotechnology, Government of India, New Delhi, for
financial support.
 
Date 2016-01-14T08:37:11Z
2016-01-14T08:37:11Z
2015
 
Type Article
 
Identifier J. Exp. Bot., 66(1): 47-57
1460-2431
http://172.16.0.77:8080/jspui/handle/123456789/539
http://jxb.oxfordjournals.org/content/66/1/47
10.1093/jxb/eru429
 
Language en_US
 
Publisher Oxford University Press