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Mapping of functional domains and characterization of the transcription factor Cph1 that mediate morphogenesis in Candida albicans

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Title Mapping of functional domains and characterization of the transcription factor Cph1 that mediate morphogenesis in Candida albicans
 
Creator Maiti, Protiti
Ghorai, Priyanka
Ghosh, Sumit
Kamthan, Mohan
Tyagi, Rakesh Kumar
Datta, Asis
 
Subject MAP kinase pathway
Transcription factor
Morphogenesis
Candida albicans
 
Description Accepted date: 15 August 2015
Cph1, a transcription factor of the Mitogen Activated Protein (MAP) kinase pathway, regulates morphogenesis in human fungal pathogen Candida albicans. Here, by following a systemic deletion approach, we have identified functional domains and motifs of Cph1 that are involved in transcription factor activity and cellular morphogenesis. We found that the N-terminal homeodomain is essential for the DNA binding activity; however, C-terminal domain and polyglutamine motif (PQ) are indispensable for the transcriptional activation function. Complementation analysis of the cph1Δ null mutant using various deletion derivatives revealed functional significance of the N- and C-terminal domains and PQ motif in filamentation process, chlamydospore formation and sensitivity to the cell wall interfering compounds. Genome-wide identification of the Cph1 binding site and quantitative RT-PCR transcript analysis in cph1Δ null mutant revealed that a number of genes which are associated with the filamentous growth, maintaining cell wall organization and mitochondrial function, and the genes of the pH response pathway are the transcriptional targets of Cph1. The data also suggest that Cph1 may function as a positive or negative regulator depending on the morphological state and physiological conditions. Moreover, differential expression of the upstream MAP kinase pathway genes in wild type and cph1Δ null mutant indicated the existence of a feedback regulation.
This work was financially supported by the Department of
Biotechnology, Ministry of Science and Technology, Government
of India. PM and PG acknowledge Council of Scientific and Industrial Research for the research fellowship. Authors thank Prof. Malcolm Whiteway, Biotechnology Research Institute, Montreal, and
Fungal Genomics Stock Center for the C. albicans strains CLJ19
and CP-1, respectively. Authors are thankful to Anil Kumar for help
in chitin analysis.
 
Date 2016-01-14T10:13:34Z
2016-01-14T10:13:34Z
2015
 
Type Article
 
Identifier Fungal Genet. Biol., 83: 45-57
1087-1845
http://172.16.0.77:8080/jspui/handle/123456789/542
http://www.sciencedirect.com/science/article/pii/S1087184515300165
10.1016/j.fgb.2015.08.004
 
Language en_US
 
Publisher Elsevier B.V.