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Investigation of genes encoding calcineurin B-like protein family in legumes and their expression analyses in chickpea (Cicer arietinum L.)

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Title Investigation of genes encoding calcineurin B-like protein family in legumes and their expression analyses in chickpea (Cicer arietinum L.)
 
Creator Meena, Mukesh K.
Ghawana, Sanjay
Sardar, Atish
Dwivedi, Vikas
Khandal, Hitaishi
Roy, Riti
Chattopadhyay, Debasis
 
Subject Cicer arietinum L.
Chickpea
Expression Analyses
 
Description Accepted date: February 20, 2015
Calcium ion (Ca2+) is a ubiquitous second messenger that transmits various internal and external signals including stresses and, therefore, is important for plants' response process. Calcineurin B-like proteins (CBLs) are one of the plant calcium sensors, which sense and convey the changes in cytosolic Ca2+-concentration for response process. A search in four leguminous plant (soybean, Medicago truncatula, common bean and chickpea) genomes identified 9 to 15 genes in each species that encode CBL proteins. Sequence analyses of CBL peptides and coding sequences (CDS) suggested that there are nine original CBL genes in these legumes and some of them were multiplied during whole genome or local gene duplication. Coding sequences of chickpea CBL genes (CaCBL) were cloned from their cDNAs and sequenced, and their annotations in the genome assemblies were corrected accordingly. Analyses of protein sequences and gene structures of CBL family in plant kingdom indicated its diverse origin but showed a remarkable conservation in overall protein structure with appearance of complex gene structure in the course of evolution. Expression of CaCBL genes in different tissues and in response to different stress and hormone treatment were studied. Most of the CaCBL genes exhibited high expression in flowers. Expression profile of CaCBL genes in response to different abiotic stresses and hormones related to development and stresses (ABA, auxin, cytokinin, SA and JA) at different time intervals suggests their diverse roles in development and plant defence in addition to abiotic stress tolerance. These data not only contribute to a better understanding of the complex regulation of chickpea CBL gene family, but also provide valuable information for further research in chickpea functional genomics.
The work was funded by the Department of
Biotechnology, Ministry of Science and Technology,
Government of India under the project 'Next
generation challenge program in chickpea genomics'
(BT/PR12919/AGR/02/676/2009). The funders had
no role in study design, data collection and analysis,
decision to publish, or preparation of the manuscript.
 
Date 2016-01-20T05:36:27Z
2016-01-20T05:36:27Z
2015
 
Type Article
 
Identifier PLoS One, 10(4): e0123640
1932-6203
http://172.16.0.77:8080/jspui/handle/123456789/544
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0123640
10.1371/journal.pone.0123640
 
Language en_US
 
Publisher PLOS