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Integrative analysis and expression profiling of secondary cell wall genes in C4 biofuel model Setaria italica reveals targets for lignocellulose bioengineering

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Title Integrative analysis and expression profiling of secondary cell wall genes in C4 biofuel model Setaria italica reveals targets for lignocellulose bioengineering
 
Creator Muthamilarasan, Mehanathan
Khan, Yusuf
Jaishankar, Jananee
Shweta, Shweta
Lata, Charu
Prasad, Manoj
 
Subject foxtail millet (Setaria italica L.)
secondary cell wall biosynthesis
lignocellulose
bioenergy grasses
genomics
comparative mapping
 
Description Accepted date: 22 October 2015
Several underutilized grasses have excellent potential for use as bioenergy feedstock due to their lignocellulosic biomass. Genomic tools have enabled identification of lignocellulose biosynthesis genes in several sequenced plants. However, the non-availability of whole genome sequence of bioenergy grasses hinders the study on bioenergy genomics and their genomics-assisted crop improvement. Foxtail millet (Setaria italica L.; Si) is a model crop for studying systems biology of bioenergy grasses. In the present study, a systematic approach has been used for identification of gene families involved in cellulose (CesA/Csl), callose (Gsl) and monolignol biosynthesis (PAL, C4H, 4CL, HCT, C3H, CCoAOMT, F5H, COMT, CCR, CAD) and construction of physical map of foxtail millet. Sequence alignment and phylogenetic analysis of identified proteins showed that monolignol biosynthesis proteins were highly diverse, whereas CesA/Csl and Gsl proteins were homologous to rice and Arabidopsis. Comparative mapping of foxtail millet lignocellulose biosynthesis genes with other C4 panicoid genomes revealed maximum homology with switchgrass, followed by sorghum and maize. Expression profiling of candidate lignocellulose genes in response to different abiotic stresses and hormone treatments showed their differential expression pattern, with significant higher expression of SiGsl12, SiPAL2, SiHCT1, SiF5H2, and SiCAD6 genes. Further, due to the evolutionary conservation of grass genomes, the insights gained from the present study could be extrapolated for identifying genes involved in lignocellulose biosynthesis in other biofuel species for further characterization.
Research on foxtail millet genomics at MP’s laboratory is funded
by the Core Grant of National Institute of Plant Genome
Research, New Delhi, India.
 
Date 2016-01-20T07:08:36Z
2016-01-20T07:08:36Z
2015
 
Type Article
 
Identifier Front. Plant Sc., 6: 965
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/550
http://journal.frontiersin.org/article/10.3389/fpls.2015.00965/abstract
10.3389/fpls.2015.00965
 
Language en_US
 
Publisher Frontiers Media S.A.