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MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated protein kinase, MPK3 in both rice and Arabidopsis

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Title MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated protein kinase, MPK3 in both rice and Arabidopsis
 
Creator Raghuram, Badmi
Sheikh, Arsheed H.
Rustagi, Yashika
Sinha, Alok Krishna
 
Subject Arabidopsis
double stranded RNA binding proteins
MAP kinase
miRNA biogenesis
miRNA microarray
protein–protein interaction
rice
 
Description Accepted date: 20 November 2014
MicroRNA (miRNA) biogenesis requires AtDRB1 (double-stranded RNA binding protein)/HYL1 (Hyponastic Leaves1) protein for processing and maturation of miRNA precursors. The AtDRB1/HYL1 protein associates with AtDCL1 (Dicer-Like1) and accurately processes primary-miRNAs (pri-mRNAs) first to precursor-miRNAs (pre-miRNAs) and finally to mature miRNAs. The dephosphorylation of AtDRB1/HYL1 protein is very important for the precise processing of miRNA precursors. The monocot model crop plant Oryza sativa encodes four orthologues of AtDRB1/HYL1 protein, the only one encoded by Arabidopsis thaliana. The present study focuses on the functionality of the O. sativa DRBs as the orthologues of AtDRB1/HYL1 by using RNA binding assays and in planta protein–protein interaction analysis. Further, mitogen-activated protein kinase MPK3 is established as the kinase phosphorylating DRB1 protein in both the model plants, O. sativa and Arabidopsis. MicroRNA microarray analysis in atmpk3 and atmpk6 mutants indicate the importance of AtMPK3 in maintaining the level of miRNAs in the plant.
BR acknowledges the Department of Biotechnology,
Government of India, while AHS thanks the Council
of Scientific and Industrial Research, India, for financial support. The authors also thank the Confocal
Microscopy Facility and the Central Instrumentation
Facility of NIPGR, New Delhi, India. The work is
supported by the core grant of the National Institute
of Plant Genome Research from the Department of
Biotechnology, Government of India.
 
Date 2016-01-21T08:29:06Z
2016-01-21T08:29:06Z
2015
 
Type Article
 
Identifier FEBS Journal, 282(3): 521-536
1742-464X
http://172.16.0.77:8080/jspui/handle/123456789/565
http://onlinelibrary.wiley.com/doi/10.1111/febs.13159/abstract
10.1111/febs.13159
 
Language en_US
 
Publisher John Wiley & Sons