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Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate

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Title Nitric oxide induces the alternative oxidase pathway in Arabidopsis seedlings deprived of inorganic phosphate
 
Creator Royo, Beatriz
Moran, Jose F.
Ratcliffe, R. George
Gupta, Kapuganti Jagadis
 
Subject Alternative oxidase
Arabidopsis thaliana
inorganic phosphate
nitric oxide
phosphate stress
reactive oxygen species
respiration
 
Description Accepted date: 11 June 2015
Phosphate starvation compromises electron flow through the cytochrome pathway of the mitochondrial electron transport chain, and plants commonly respond to phosphate deprivation by increasing flow through the alternative oxidase (AOX). To test whether this response is linked to the increase in nitric oxide (NO) production that also increases under phosphate starvation, Arabidopsis thaliana seedlings were grown for 15 d on media containing either 0 or 1mM inorganic phosphate. The effects of the phosphate supply on growth, the production of NO, respiration, the AOX level and the production of superoxide were compared for wild-type (WT) seedlings and the nitrate reductase double mutant nia. Phosphate deprivation increased NO production in WT roots, and the AOX level and the capacity of the alternative pathway to consume electrons in WT seedlings; whereas the same treatment failed to stimulate NO production and AOX expression in the nia mutant, and the plants had an altered growth phenotype. The NO donor S-nitrosoglutathione rescued the growth phenotype of the nia mutants under phosphate deprivation to some extent, and it also increased the respiratory capacity of AOX. It is concluded that NO is required for the induction of the AOX pathway when seedlings are grown under phosphate-limiting conditions.
This work was supported by research grants (AGL2010-16167; AGL2014-
52396-P) from the Spanish Ministry of Economy and Competitiveness
(MINECO) to JFM, an FPI fellowship from MINECO (BR), and a Marie
Curie Intra-European Fellowship for Career Development within the 7th
European Community Framework Programme (KJG and RGR). We thank
Mr John Baker for photography.
 
Date 2016-01-21T09:03:18Z
2016-01-21T09:03:18Z
2015
 
Type Article
 
Identifier J. Exp. Bot., 66(20): 6273-6280
1460-2431
http://172.16.0.77:8080/jspui/handle/123456789/568
http://jxb.oxfordjournals.org/content/66/20/6273.long
10.1093/jxb/erv338
 
Language en_US
 
Publisher Oxford University Press