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Simultaneous isolation of root and leaf mitochondria from Arabidopsis

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Title Simultaneous isolation of root and leaf mitochondria from Arabidopsis
 
Creator Gupta, Kapuganti Jagadis
Ewald, Ralph
 
Subject aerobic organisms
Arabidopsis
bulky tissues
chlorophyll
leaf mitochondria
root mitochondria isolation
 
Description Accepted date: 22 MAY 2015
In all aerobic organisms mitochondria generate ATP via oxidative phosphorylation. For bulky tissues such as potato tubers and cauliflower and for larger crop plants such as tobacco, pea, soybean or etiolated seedlings it is possible to get a good yield of mitochondria. However, for tiny model plants like Arabidopsis it is very difficult to get sufficient quantities of mitochondria for various studies. Moreover, for comparative studies it is very important to isolate leaf and root mitochondria. Due to the lack of chlorophyll, root mitochondria isolation is often an easy task. Leaf mitochondria isolation has the advantage that higher amounts of tissues can be obtained from the plants in comparison to root material, but chlorophyll contamination can be a problem. This chapter describes how to isolate root mitochondria with sufficiently high yields, and how to obtain chlorophyll-free leaf mitochondria simultaneously.
 
Date 2016-01-21T10:39:25Z
2016-01-21T10:39:25Z
2015
 
Type Book chapter
 
Identifier In: Gupta KJ, Mur LAJ, Neelwarne B (eds.), Alternative Respiratory Pathways in Higher Plants. John Wiley & Sons, UK, pp 359-365
9781118789971
http://172.16.0.77:8080/jspui/handle/123456789/572
http://onlinelibrary.wiley.com/doi/10.1002/9781118789971.ch25/summary
10.1002/9781118789971.ch25
 
Language en_US
 
Publisher John Wiley & Sons