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Genome-wide survey and comprehensive expression profiling of Aux/IAA gene family in chickpea and soybean

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Title Genome-wide survey and comprehensive expression profiling of Aux/IAA gene family in chickpea and soybean
 
Creator Singh, Vikash K.
Jain, Mukesh
 
Subject gene family
Aux/IAA
chickpea
soybean
gene duplication
transposed duplication
gene expression
abiotic stress
 
Description Accepted date: 12 October 2015
Auxin plays a central role in many aspects of plant growth and development. Auxin/Indole-3-Acetic Acid (Aux/IAA) genes cooperate with several other components in the perception and signaling of plant hormone auxin. An investigation of chickpea and soybean genomes revealed 22 and 63 putative Aux/IAA genes, respectively. These genes were classified into six subfamilies on the basis of phylogenetic analysis. Among 63 soybean Aux/IAA genes, 57 (90.5%) were found to be duplicated via whole genome duplication (WGD)/segmental events. Transposed duplication played a significant role in tandem arrangements between the members of different subfamilies. Analysis of Ka/Ks ratio of duplicated Aux/IAA genes revealed purifying selection pressure with restricted functional divergence. Promoter sequence analysis revealed several cis-regulatory elements related to auxin, abscisic acid, desiccation, salt, seed, and endosperm, indicating their role in development and stress responses. Expression analysis of chickpea and soybean Aux/IAA genes in various tissues and stages of development demonstrated tissue/stage specific differential expression. In soybean, at least 16 paralog pairs, duplicated via WGD/segmental events, showed almost indistinguishable expression pattern, but eight pairs exhibited significantly diverse expression patterns. Under abiotic stress conditions, such as desiccation, salinity and/or cold, many Aux/IAA genes of chickpea and soybean revealed differential expression. qRT-PCR analysis confirmed the differential expression patterns of selected Aux/IAA genes in chickpea. The analyses presented here provide insights on putative roles of chickpea and soybean Aux/IAA genes and will facilitate elucidation of their precise functions during development and abiotic stress responses.
This work was financially supported by the Department of
Biotechnology, Government of India, New Delhi, under the
Challenge Programme on Chickpea Functional Genomics.
 
Date 2016-01-22T10:18:05Z
2016-01-22T10:18:05Z
2015
 
Type Article
 
Identifier Front. Plant Sc., 6: 918
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/582
http://journal.frontiersin.org/article/10.3389/fpls.2015.00918/abstract
10.3389/fpls.2015.00918
 
Language en_US
 
Publisher Frontiers Media S.A.