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Genome-wide analysis and expression profiling suggest diverse roles of GH3 genes during development and abiotic stress responses in legumes

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Title Genome-wide analysis and expression profiling suggest diverse roles of GH3 genes during development and abiotic stress responses in legumes
 
Creator Singh, Vikash K.
Jain, Mukesh
Garg, Rohini
 
Subject gene family
GH3 genes
legumes
RNA-seq
homology modeling
substrate specificities
 
Description Accepted date: 18 December 2014
Growth hormone auxin regulates various cellular processes by altering the expression of diverse genes in plants. Among various auxin-responsive genes, GH3 genes maintain endogenous auxin homeostasis by conjugating excess of auxin with amino acids. GH3 genes have been characterized in many plant species, but not in legumes. In the present work, we identified members of GH3 gene family and analyzed their chromosomal distribution, gene structure, gene duplication and phylogenetic analysis in different legumes, including chickpea, soybean, Medicago, and Lotus. A comprehensive expression analysis in different vegetative and reproductive tissues/stages revealed that many of GH3 genes were expressed in a tissue-specific manner. Notably, chickpea CaGH3-3, soybean GmGH3-8 and -25, and Lotus LjGH3-4, -5, -9 and -18 genes were up-regulated in root, indicating their putative role in root development. In addition, chickpea CaGH3-1 and -7, and Medicago MtGH3-7, -8, and -9 were found to be highly induced under drought and/or salt stresses, suggesting their role in abiotic stress responses. We also observed the examples of differential expression pattern of duplicated GH3 genes in soybean, indicating their functional diversification. Furthermore, analyses of three-dimensional structures, active site residues and ligand preferences provided molecular insights into function of GH3 genes in legumes. The analysis presented here would help in investigation of precise function of GH3 genes in legumes during development and stress conditions.
This work was funded by the Department of Biotechnology, Government of India, under the Next Generation
Challenge Programme on Chickpea Genomics (grant number BT/PR12919/AGR/02/676/2009 from 2009 to 2014) to Mukesh Jain. Rohini Garg acknowledges INSPIRE faculty award from the
Department of Science and Technology, Government of India.
Vikash K. Singh acknowledges the award of research fellowship
from the Department of Biotechnology, Government of India.
 
Date 2016-01-22T10:28:04Z
2016-01-22T10:28:04Z
2015
 
Type Article
 
Identifier Front. Plant Sc., 5: 789
1664-462X
http://172.16.0.77:8080/jspui/handle/123456789/583
http://journal.frontiersin.org/article/10.3389/fpls.2014.00789/abstract
10.3389/fpls.2014.00789
 
Language en_US
 
Publisher Frontiers Media S.A.