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Cloning and expression of gamma carbonic anhydrase from Serratia sp. ISTD04 for sequestration of carbon dioxide and formation of calcite

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Title Cloning and expression of gamma carbonic anhydrase from Serratia sp. ISTD04 for sequestration of carbon dioxide and formation of calcite
 
Creator Srivastava, Shaili
Bharti, Randhir Kumar
Verma, Praveen K.
Thakur, Indu shekhar
 
Subject CO2 sequestration
Carbonic anhydrase
FTIR
XRD
Serratia sp.
 
Description Accepted date: 27 January 2015
Bacterial strains isolated from marble mines rock and enriched in the chemostat culture with different concentrations of sodium bicarbonate. The enriched consortium had six bacterial isolates. One of bacterium isolate showed carbonic anhydrase (CA) activity by catalyzing the reversible hydration reaction of carbon dioxide to bicarbonate. The bacterium was identified as Serratia sp. by 16S rRNA sequence analysis. The carbonic anhydrase gene from Serratia sp. was found to be homologous with gamma carbonic anhydrase. The carbonic anhydrase gene was cloned in PET21b(+) and expressed it in recombinant Escherichia coli BL21 (DE3) with His-tag at the C-terminus. The recombinant protein was purified efficiently by using one-step nickel affinity chromatography. Expected size of carbonic anhydrase was approximately 29 kDa in SDS-PAGE gel. Recombinant carbonic anhydrase enzyme was used for biomineralization-based conversion of atmospheric CO2 into valuable calcite minerals. The calcification was confirmed by using XRD, FTIR, EDX and SEM analysis.
The author is grateful to Department of Science and Technology
Government of India, New Delhi, for providing start up research
fund in form of project (Srivastava, S). The Instrumentation like XRD, FTIR and SEM-EDX facility was provided by AIRF, Jawaharlal
Nehru University, New Delhi, India.
 
Date 2016-01-25T08:34:12Z
2016-01-25T08:34:12Z
2015
 
Type Article
 
Identifier Bioresource Technol., 188: 209-213
0960-8524
http://172.16.0.77:8080/jspui/handle/123456789/584
http://www.sciencedirect.com/science/article/pii/S0960852415001285
10.1016/j.biortech.2015.01.108
 
Language en_US
 
Publisher Elsevier B.V.